中国水产科学2023，Vol.30Issue(9)：1112-1126,15.DOI:10.12264/JFSC2023-0139
Cyt b和12S rRNA基因条形码在灯笼鱼科鱼类物种鉴定中的应用
Identification analysis of DNA barcoding based on mitochondrial Cyt b and 12S rRNA genes in Myctophidae fishes
摘要
Abstract
The Myctophidae family is widely distributed in mesopelagic oceans worldwide and plays a significant role in the pelagic food chain due to its vertical migratory behavior.Myctophidae are diverse and the morphological differences between genera are not obvious.Therefore,the use of molecular markers for Myctophidae identification would be advantageous.In this study,we amplified 56 Myctophidae samples collected from the Northwest Pacific using the Cyt b and 12S rRNA genes.These amplified sequences were subsequently used for phylogenetic analyses alongside sequences from 29 other Myctophidae species in 18 genera from the GenBank database.We explored the applicability of Cyt b and 12S rRNA genes for identifying Myctophidae species.The results indicate that the 56 samples belonged to 6 species:Ceratoscopelus warmingii,Symbolophorus californiensis,Myctophum asperum,Tarletonbeania crenularis,Notoscopelus japonicus,and Notoscopelus sp.The intra-specific and inter-specific genetic distances based on the Cyt b gene were more pronounced than those based on the 12S rRNA gene.The difference between the average inter-specific and intra-specific genetic distances of Cyt b and 12S rRNA gene was 25 and 26,respectively.These results indicated that both genes meet the basic requirements for DNA barcodes.Phylogenetic analysis evidenced that the six Myctophidae species each formed an independent clade based on Cyt b and 12S rRNA gene sequences.However,in the evolutionary tree constructed based on the Cyt b gene,each fish clustered more closely with sequences in the GenBank database.In summary,both Cyt b and 12S rRNA genes could be used as DNA barcodes for identifying Myctophidae species,amd the Cyt b gene was particularly suitable for analyzing phylogenetic relationships.关键词
灯笼鱼科/Cyt b基因/12S rRNA基因/物种鉴定/系统进化分析Key words
Myctophidae/cytochrome b gene/12S rRNA gene/species identification/phylogenetic analysis分类
农业科技引用本文复制引用
刘乙蒙,刘洋,刘必林,俞晔伟,宋成辉,王丛丛..Cyt b和12S rRNA基因条形码在灯笼鱼科鱼类物种鉴定中的应用[J].中国水产科学,2023,30(9):1112-1126,15.基金项目
农业农村部全球渔业资源调查监测评估(公海渔业资源综合科学调查)专项 （公海渔业资源综合科学调查）
国家远洋渔业工程技术研究中心、大洋渔业资源可持续开发教育部重点实验室、农业农村部大洋渔业可持续利用重点实验室开放基金项目(A1-2006-23-200210). （A1-2006-23-200210）
