智慧农业导刊2024,Vol.4Issue(5):55-58,4.DOI:10.20028/j.zhnydk.2024.05.014
北沙参种子内源抑制物质活性研究
摘要
Abstract
The effects of seed and pericarp isolation of Radix Glehniae(aks.CoasiaI GIehnia Root)on seed germination and seedlings of Chinese cabbage were studied to explore the activity of endogenous inhibitors in seeds of Radix Glehniae.The extracts from different parts of the seeds of Radix Glehniae were separated by systematic solvent method,and the petroleum ether phase,dichloromethane phase,ethyl acetate phase,n-butanol phase,methanol phase and water phase were separated,and the germination experiments were carried out relative to Chinese cabbage seeds.The biological activity of endogenous inhibitors in Radix Glehniae seeds was determined.The results showed that:①The separation of seeds and pericarps of Radix Glehniae had a certain inhibitory effect on the germination of Chinese cabbage seeds,in which methanol phase was the strongest,followed by ethyl acetate phase.Compared with the control group,the germination index of each treatment group decreased to a certain extent,and the average germination days of methanol phase was significantly longer than that of other treatments.②The separated phases of seeds and pericarps of Radix Glehniae showed obvious differences in inhibiting root length and bud length of Chinese cabbage seedlings,among which methanol phase was the most significant,followed by ethyl acetate phase.The results were consistent with the germination index.The isolation of different parts of the seeds of Radix Glehniae had different inhibitory effects on the seed germination and seedlings of Chinese cabbage,which indicated that the seeds and pericarps of Radix Pseudostellariae contained highly active inhibitors,which mainly existed in methanol phase and ethyl acetate phase.The endogenous inhibitor in the seeds of Radix Glehniae is the main reason for its dormancy.关键词
蒙药/北沙参/种子休眠/内源抑制物/生物活性Key words
Mongolian medicine/Radix Glehniae/seed dormancy/endogenous inhibitor/biological activity分类
医药卫生引用本文复制引用
杨灵珍,红艳,辛颖,双叶,乌日力嘎..北沙参种子内源抑制物质活性研究[J].智慧农业导刊,2024,4(5):55-58,4.基金项目
内蒙古自治区直属高校基本科研业务费项目(GXKY22140) (GXKY22140)
内蒙古自治区研究生科研创新项目(S20231202Z) (S20231202Z)
内蒙古民族大学中药学(蒙药学)重点学科建设项目(ZYX004) (蒙药学)