中药材2023，Vol.46Issue(8)：1865-1869,5.DOI:10.13863/j.issn1001-4454.2023.08.004
浙麦冬愈伤组织的诱导与再生植株的获得
Induction of Callus and Acquisition of Regenerative Plants of Ophiopogon japonicus from Zhejiang
摘要
Abstract
Objective:To establish tissue culture and rapid propagation system of Ophiopogon japonicus from Zhejiang,so as to pro-vide certain theoretical basis and technical support for its scientific and large-scale production.Methods:Taking the leaf base of"Zhejiang Ophiopogon japonicus No.1"as an explant,the effects of plant growth regulators on inducing callus,clump bud differentiation and adventitious root formation were studied,and the seedlings were refined and transplanted.Results:The optimal medium for callus in-duction of"Zhejiang Ophiopogon japonicus No.1"was MS with 2.0 mg/L KT,0.5 mg/L NAA,30.0 g/L sugar,2.5 g/L agar,2.0 g/L activated carbon,the induction rate was 92.22%;The optimal medium for callus differentiation adventitious buds was MS with 4.0 mg/L KT,0.5 mg/L NAA,30.0 g/L sugar,2.5 g/L agar,2.0 g/L activated carbon,and the adventitious bud differentiation rate was 88.89%.The optimal rooting medium was 1/2 MS with 0.5 mg/L NAA,30.0 g/L sugar,2.5 g/L agar,and the rooting rate was 96.67%;The survival rate of tissue culture seedlings was 89.00%after two weeks of domestication.Conclusion:The rapid propagation technology of"Zhejiang Ophiopogon japonicus No.1"tissue culture is established.关键词
浙麦冬/快繁/无菌苗/组织培养Key words
Ophiopogon japonicus(L.f.)Ker-Gawl.from Zhejiang/Fast and profane/Sterile seedlings/Tissue culture分类
中医学引用本文复制引用
俞春英,徐建中,王志安,江建铭..浙麦冬愈伤组织的诱导与再生植株的获得[J].中药材,2023,46(8):1865-1869,5.基金项目
国家重点研发计划中医药现代化研究专项(2017YFC1700703) （2017YFC1700703）
浙江省农业新品种选育重大科技专项(2021C02074) （2021C02074）
国家现代农业产业技术体系资助(CARS-21) （CARS-21）
浙江省特色中药资源保护与创新利用重点实验室项目(2021E10013) （2021E10013）
省部共建秦药特色资源研究与开发国家重点实验室(培育)开放课题(QY202103). （培育）
