中药材2023，Vol.46Issue(8)：1912-1918,7.DOI:10.13863/j.issn1001-4454.2023.08.012
基于特征图谱和色彩图像技术的生地黄与熟地黄的差异比较研究
Study on the Difference Comparison of Rehmanniae Radix and Rehmanniae Radix Praeparata Based on Characteristic Spectrum and Color Image Technology
摘要
Abstract
Objective:To compare the differences between Rehmanniae Radix and Rehmanniae Radix Praeparata by using UPLC and digital imaging technology.Methods:The chromatograms and chromaticity value of Rehmanniae Radix and Rehmanniae Radix Pra-eparata were analyzed by using multivariate statistical analysis methods,such as similarity evaluation,paired sample t-test,Wilcoxon sign average rank test,orthogonal partial least squares discriminant analysis(OPLS-DA),cluster analysis,etc.Results:18 and 20 com-mon peaks were identified by the established UPLC characteristic spectrum of Rehmanniae Radix and Rehmanniae Radix Praeparata,respectively.Among them,peaks 19(5-hydroxymethylfurfural),20 appeared after the processing of Rehmanniae Radix.The peak areas of peaks 3 to 9 and peaks 11 to 18 were significantly different between Rehmanniae Radix and Rehmanniae Radix Praeparata.The OPLS-DA results showed that the peaks 1,2,4,19 were the main factors in the difference of Rehmanniae Radix and Rehmanniae Radix Praeparata.The ΔE* between Rehmanniae Radix and Rehmanniae Radix Praeparata was 16.47 to 31.11,indicating that raw and pro-cessed products could be clearly identified.The L*,a*,b* and E* could be used as one of the distinguishing bases between Reh-manniae Radix and Rehmanniae Radix Praeparata.Conclusion:The UPLC characteristic spectrum method of Rehmanniae Radix and Re-hmanniae Radix Praeparata established in this study is stable and reliable,and it can be used to distinguish Rehmanniae Radix and Re-hmanniae Radix Praeparata by combining the chromaticity value.关键词
地黄/熟地黄/特征图谱/色度值/多元统计分析方法Key words
Rehmanniae Radix/Rehmanniae Radix Praeparata/Characteristic spectrum/Chromaticity value/Multivariate statisti-cal analysis method分类
医药卫生引用本文复制引用
纪玉华,王文丽,杨赞,甘力帆,徐杰,邓韬,张志鹏..基于特征图谱和色彩图像技术的生地黄与熟地黄的差异比较研究[J].中药材,2023,46(8):1912-1918,7.基金项目
中华人民共和国工业和信息化部科技司2022年产业技术基础公共服务平台项目(2022-230-221) （2022-230-221）
