中药材2023，Vol.46Issue(8)：1924-1930,7.DOI:10.13863/j.issn1001-4454.2023.08.014
鹅不食草化学成分及其抗炎活性研究
Study on Chemical Constituents of Centipeda minima and Anti-inflammatory Activity
摘要
Abstract
Objective:To study the chemical constituents and anti-inflammatory activity of Centipeda minima.Methods:The chemi-cal constituents of the 80%ethanol extract of Centipeda minima were isolated and purified by various column chromatography and pre-parative liquid methods,and the structures were identified on the basis of NMR and MS spectral analysis.The anti-inflammatory activity of the compounds was evaluated by detecting the release of inflammatory mediators NO from mice macrophages(RAW264.7)induced by lipopolysaccharide(LPS).Results:Twenty-two compounds were isolated from Centipeda minima and identified as taraxasteryl acetate(1),squalene(2),lupenone(3),taraxasterol(4),friedelin(5),taraxasterone(6),pseudotaraxasterol acetate(7),lupeol acetate(8),β-sitosterol(9),9-hydroxythymol(10),8-hydroxy-9-isobutyryloxy-10(2)-methylbutyryloxythymol(11),8,10-dihydroxy-9-isobutyryloxy-thymol(12),E-pcoumaryl-alcohol ethyl ether(13),2-hydroxy-4-methy-lbenzoic acid(14),3-deuteriomethyl-5-methyl-2,3-dihydro-benzofyran(15),1,3-dimethoxybenzene(16),vanillic acid(17),ethyl-2-(3,4-dihydroxyphenyl)acetate(18),5-hydroxy-6,8-dime-thoxycoumarin(19),indazole(20),patriscabratine(21),5-(hydroxymethyl)-2,4,4-trimethyl-2-cyclohexen-1-one(22),respectively.Conclusion:Among them,compounds 2,3,5,6,13 to 22 are isolated from Centipeda genus for the first time,compounds 10 to 15,17,19 to 22 show strong anti-inflammatory activity.关键词
鹅不食草/化学成分/抗炎活性Key words
Centipeda minima(L.)A.Br.et Aschers./Chemical constituents/Anti-inflammatory activity分类
医药卫生引用本文复制引用
杨柳,高奇,周浪,李齐激,杨娟,杨艳..鹅不食草化学成分及其抗炎活性研究[J].中药材,2023,46(8):1924-1930,7.基金项目
贵州省科技创新能力建设专项(黔科合服企[2020]4013号) （黔科合服企[2020]4013号）
贵州省教育厅高等学校特色重点实验室(黔教合KY字[2020]018号) （黔教合KY字[2020]018号）
