中药材2023，Vol.46Issue(8)：2016-2022,7.DOI:10.13863/j.issn1001-4454.2023.08.030
苦豆碱通过抑制PI3K/AKT/mTOR信号通路调控前列腺癌细胞自噬与凋亡
Study on the Aloperine Regulating Autophagy and Apoptosis of Prostate Cancer Cells by Inhibiting PI3K/AKT/mTOR Signaling Pathway
摘要
Abstract
Objective:To investigate the inhibitory effect and its possible mechanism of aloperine on PC3 and LNCaP cells of pros-tatic cancer.Methods:Different concentrations of aloperine(0,25,50,100,200,400,800,1 600 μmol)were used to treat PC3 and LN-CaP cells of prostate cancer.The proliferative activities of PC3 and LNCaP cells were detected by CCK-8 assay and clonal formation as-say.Morphological changes of cells were observed by optical microscope.The apoptosis rate was detected by flow cytometry.The expres-sion of autophagy index LC3Ⅱ was detected by immunofluorescence.The expressions of apoptosis and autophagy related proteins Caspase-3,Bax,Bcl-2,LC3Ⅱ,Beclin 1,P62 and key proteins in PI3K/AKT/mTOR pathway were detected by Western Blot.Results:The results of CCK-8 and clonal formation experiments showed that aloperine inhibited the activities of PC3 and LNCaP cells in a con-centration-dependent manner.Compared with the normal control group(0.1%DMSO),the cell morphology of PC3 and LNCaP cells treated with 100 and 200 μmol aloperine was significantly changed.Flow cytometry results showed that the apoptosis rate was increased.Western Blot results showed that the expressions of apoptosis-related proteins Caspase-3 and Bax were significantly increased,and the expression of Bcl-2 was significantly decreased.The expressions of autophagy related protein Beclin 1 and the ratio of LC3Ⅱ/LC3Ⅰwere significantly increased,while the expression of P62 was decreased.The ratio of PI3K pathway related proteins p-PI3K/PI3K,p-AKT/AKT and p-mTOR/mTOR decreased.Conclusion:Aloperine induces autophagy and apoptosis of PC3 and LNCaP cells of prostate cancer through PI3K/AKT/mTOR pathway and inhibits cell proliferation.关键词
苦豆碱/前列腺癌/PI3K/AKT/mTOR/自噬Key words
Aloperine/Prostate cancer/PI3K/AKT/mTOR/Autophagy分类
医药卫生引用本文复制引用
安康杰,李旭,杨尉鑫,聂黎虹,李亚杰,赵瑞宁..苦豆碱通过抑制PI3K/AKT/mTOR信号通路调控前列腺癌细胞自噬与凋亡[J].中药材,2023,46(8):2016-2022,7.基金项目
宁夏自然科学基金资助项目(2022AAC03506,2022AAC03160) （2022AAC03506,2022AAC03160）
