中国中西医结合杂志2024，Vol.44Issue(2)：216-221,6.DOI:10.7661/j.cjim.20231227.265
超临界流体强化溶液快速分散技术制备三七皂苷脂质体性质研究
Properties Study of Panax Notoginseng Saponins Liposome Prepared by Solution Enhanced Dispersion by Supercritical Fluid
摘要
Abstract
Objective To observe the pharmaceutical characteristics of Panax notoginseng saponins liposome(PNS-Lip)prepared by solution enhanced dispersion by supercritical fluid(SEDS),and compare with conventional liposome preparation methods.Methods PNS-Lip was prepared by SEDS,thin-film dispersion method(TFD)and reversed-phase evaporation method(RPE).The appearance and morphology of different PNS-Lip were observed by transmission electron microscope;The particle size and potential of PNS-Lip were detected.The encapsulation rate(EE)and drug load(DL)were determined by ultrafiltration method.Leakage rate(LR)was used to evaluate the stability of the preparation.The dissolution test was performed in vitro.Results The PNS-Lip prepared by different preparation methods showed light blue opalescence,smooth surface,round shape and double-layer film structure.Compared with PNS-Lip-RPE,the particle size,polydispersity index(PDI)and Zeta potential of PNS-Lip-TFD and PNS-Lip-SEDS significantly decreased,and the LR at 60,120,180,240,300 and 360 s also significantly decreased,the EE and DL significantly increased(P＜0.05,P＜0.01).In addition,the DR of PNS-Lip-SEDS significantly decreased in 420 s(P＜0.05).Compared with PNS-Lip-TFD,the particle size,PDI and Zeta potential of PNS-Lip-SEDS significantly decreased,and LR also significantly decreased at 180,240,300,360 and 420 s,while EE and DL increased(P＜0.05,P＜0.01).PNS-Lip-RPE and PNS-Lip-TFD had sudden release in the initial stage,and then continued to release slowly.However,there was no sudden release of PNS-Lip-SEDS with a more obvious sustained release effect which reached dissolution equilibrium after 48 h.Conclusion As a new granulating technology,SEDS has certain advantages compared with the conventional liposome preparation method,and it has broad application prospects in the field of nano-drug delivery system.关键词
三七皂苷/脂质体/超临界流体强化溶液快速分散技术/体外溶出/中药单体Key words
Panax notoginseng saponins/liposome/solution enhanced dispersion by supercritical fluid/in vitro dissolution/Chinese medicine monomers引用本文复制引用
赖志昆,胡晓贞,翁嘉灏,冯其茂,张晓嫣..超临界流体强化溶液快速分散技术制备三七皂苷脂质体性质研究[J].中国中西医结合杂志,2024,44(2):216-221,6.基金项目
上海市卫生健康委员会项目(No.20204Y0260) （No.20204Y0260）
上海市科学技术委员会项目(No.21Y11920700) （No.21Y11920700）
国家自然科学青年基金资助项目(No.82104758) （No.82104758）
