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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

Gyeong-Min Gim Kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-Koo Yi Jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son Soo-Young Yum Won-Wu Lee Goo Jang

Journal of Animal Science and Biotechnology2024,Vol.15Issue(1):P.456-462,7.
Journal of Animal Science and Biotechnology2024,Vol.15Issue(1):P.456-462,7.DOI:10.1186/s40104-023-00902-8

Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

Gyeong-Min Gim 1Kyeong-Hyeon Eom 1Dong-Hyeok Kwon 2Dae-Jin Jung 3Dae-Hyun Kim 3Jun-Koo Yi 3Jae-Jung Ha 3Ji-Hyun Lee 4Seong-Beom Lee 4Woo-Jae Son 4Soo-Young Yum 4Won-Wu Lee 4Goo Jang5

作者信息

  • 1. Laboratory of Theriogenology and Biotechnology,Department of Veterinary Clinical Science,College of Veterinary Medicine and the Research Institute of Veterinary Science,Seoul National University,Seoul,Republic of Korea LARTBio Co.,Ltd.,Seoul,Republic of Korea
  • 2. Laboratory of Theriogenology and Biotechnology,Department of Veterinary Clinical Science,College of Veterinary Medicine and the Research Institute of Veterinary Science,Seoul National University,Seoul,Republic of Korea
  • 3. Gyeongsangbukdo Livestock Research Institute,Yeongju,Republic of Korea
  • 4. LARTBio Co.,Ltd.,Seoul,Republic of Korea
  • 5. Laboratory of Theriogenology and Biotechnology,Department of Veterinary Clinical Science,College of Veterinary Medicine and the Research Institute of Veterinary Science,Seoul National University,Seoul,Republic of Korea LARTBio Co.,Ltd.,Seoul,Republic of Korea Comparative Medicine Disease Research Center,Seoul National University,Seoul,Republic of Korea
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摘要

关键词

Beta-lactoglobulin/Cattle/CRISPR-Cas9/Electroporation/Knockout/MSTN/PRNP

分类

农业科技

引用本文复制引用

Gyeong-Min Gim,Kyeong-Hyeon Eom,Dong-Hyeok Kwon,Dae-Jin Jung,Dae-Hyun Kim,Jun-Koo Yi,Jae-Jung Ha,Ji-Hyun Lee,Seong-Beom Lee,Woo-Jae Son,Soo-Young Yum,Won-Wu Lee,Goo Jang..Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation[J].Journal of Animal Science and Biotechnology,2024,15(1):P.456-462,7.

基金项目

financially supported by the National Research Foundation of Korea(NRF-2021R1A5A1033157 for SRC program:382 Comparative medicine Disease Research Center,NRF-2021R1F1A105195313) (NRF-2021R1A5A1033157 for SRC program:382 Comparative medicine Disease Research Center,NRF-2021R1F1A105195313)

the Research Institute of Veterinary Science,the BK21 Four for Future Veterinary Medicine Leading Education and Research Center,and a Seoul National University(SNU)grant(#550e2020005)。 (SNU)

Journal of Animal Science and Biotechnology

OACSTPCD

1674-9782

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