大豆科学2024，Vol.43Issue(1)：13-20,8.DOI:10.11861/j.issn.1000-9841.2024.01.0013
代表性春大豆种质资源叶片蔗糖含量全基因组关联分析
Genome-wide Association Study of Sucrose Content in Leaves of Representative Spring Soybean Germplasm Resources
摘要
Abstract
Sucrose is the main form of plant sugar source and storage transportation,and is an important energy substance in plant growth.In this study,the 196 natural populations consisting of domestic and foreign varieties that had been re-sequenced were used as test materials,and sucrose content of seedling stage in leaves in this population was measured,and a genome wide association analysis was performed with a mixed linear mode.The results showed that 5 significantly related SNP loci were screened according to the threshold,which were located on chromosomes 15 and 20,and 27 related genes were searched in the interval of 100 kb upstream and 100 kb downstream of the SNP loci.The 5 candidate genes that might be related to sucrose content in soybean leaves were screened by GO functional enrichment analysis,KEGG metabolic pathway enrichment analysis and gene function annotation.Three genes related to sucrose content in soybean leaves,including Glyma.15G023800,Glyma.15G024000 and Glyma.15G024600,were identified by relative expression analysis.The results of this study provide a theoretical reference to investigate the genetic mechanism of sucrose content in soybean leaves.关键词
大豆/叶片/蔗糖/全基因组关联分析/候选基因Key words
soybean/leaves/sucrose/genome wide association analysis/candidate gene引用本文复制引用
王象然,张大勇,郑伟,张振宇,徐杰飞,赵星棋,孙长恒,吴雨恒..代表性春大豆种质资源叶片蔗糖含量全基因组关联分析[J].大豆科学,2024,43(1):13-20,8.基金项目
国家重点研发计划(2021YFD1201102) （2021YFD1201102）
黑龙江省揭榜挂帅项目(2021ZXJ05B02). （2021ZXJ05B02）
