大豆GmNF-YA13互作蛋白的筛选及鉴定OA北大核心CSTPCD

Soybean(Glycine max)GmNF-YA13 protein is a nuclear transcription factor Y(NF-Y)that plays a crucial role in drought and high salinity responses.To investigate its mechanisms of drought resistance and salt tolerance,and identify the interacting proteins of GmNF-YA13,a pGBKT7-GmNF-YA13 bait vector was constructed.The soybean yeast library was screened using yeast two-hybrid system,and verified by staining with X-α-gal.The result showed that:85 positive clones were obtained after sequencing analysis,36 candidate interacting proteins were identified.Functional prediction showed that these interacting proteins are mainly involved in growth and development,stress responses,energy metabolism,transcription regulation,and signal transduction processes.GmUVR8,GmCMI41,GmFbox13,and GmFBA were selected for one-on-one validation with the bait vector pGBKTI7-GmNF-YA13.The results revealed that only GmFBA could interact with GmNF-YA13,indicating that the function of GmNF-YA13 requires the involvement of GmFBA.These findings lay the foundation for further research on the NF-YA stress resistance molecular network.