大豆科学2024，Vol.43Issue(1)：21-28,8.DOI:10.11861/j.issn.1000-9841.2024.01.0021
大豆GmNF-YA13互作蛋白的筛选及鉴定
Screening and Identification of Interacting Proteins of GmNF-YA13 in Soybean
摘要
Abstract
Soybean(Glycine max)GmNF-YA13 protein is a nuclear transcription factor Y(NF-Y)that plays a crucial role in drought and high salinity responses.To investigate its mechanisms of drought resistance and salt tolerance,and identify the interacting proteins of GmNF-YA13,a pGBKT7-GmNF-YA13 bait vector was constructed.The soybean yeast library was screened using yeast two-hybrid system,and verified by staining with X-α-gal.The result showed that:85 positive clones were obtained after sequencing analysis,36 candidate interacting proteins were identified.Functional prediction showed that these interacting proteins are mainly involved in growth and development,stress responses,energy metabolism,transcription regulation,and signal transduction processes.GmUVR8,GmCMI41,GmFbox13,and GmFBA were selected for one-on-one validation with the bait vector pGBKTI7-GmNF-YA13.The results revealed that only GmFBA could interact with GmNF-YA13,indicating that the function of GmNF-YA13 requires the involvement of GmFBA.These findings lay the foundation for further research on the NF-YA stress resistance molecular network.关键词
核转录因子/GmNF-YA13/酵母双杂交/互作蛋白Key words
nuclear transcription factor/GmNF-YA13/yeast two-hybrid/interacting protein引用本文复制引用
刘灿,于月华,倪志勇..大豆GmNF-YA13互作蛋白的筛选及鉴定[J].大豆科学,2024,43(1):21-28,8.基金项目
国家自然科学基金(32160446) （32160446）
新疆维吾尔自治区自然科学基金(2022D01E14,2021D01A93). （2022D01E14,2021D01A93）
