大豆科学2024，Vol.43Issue(1)：29-34,6.DOI:10.11861/j.issn.1000-9841.2024.01.0029
耐盐转基因大豆事件AtARA6-A001外源插入片段侧翼序列及其应用
Flanking Sequence of Exogenous Insertion in Salt-tolerant Transgenic Soybean Event AtARA6-A001 and Its Application
摘要
Abstract
AtARA6 gene from Arabidopsis thaliana was introduced into soybean receptor Shennong 9 by Agrobacterium-mediated transformation of soybean cotyledon nodes,obtaining the salt-tolerant transgenic soybean event AtARA6-A001.To identify the molecular characteristics and setting up a detection method of this transgenic soybean material,we could further accelerate the biosafety evaluation of this transgenic event in the future.In this study,the copy number of exogenous gene of the homozygous transgenic soybean AtARA6-A001 was identified by Southern hybridization as well as the position and direction of the insertion site.Meanwhile,the left and right flanking sequences of exogenous T-DNA were obtained by PCR amplification.Based on these data,a specific qualitative PCR method for detecting AtARA6 gene salt-tolerant soybean A001 event was established.This method has high specificity,and can quickly identify transgenic soybean events,including AtARA6-A001 parents,derivatives,or varieties,as well as plants,tissues,seeds and products for specific detection,achieving effective supervision and management of transgenic soybeans by technical support.关键词
大豆/转基因/侧翼序列/重测序/定性PCR检测Key words
soybean/transgene/flanking sequence/resequencing/qualitative PCR detection引用本文复制引用
孙星邈,侯云龙,王英哲,关诗宇,邱红梅,张玲..耐盐转基因大豆事件AtARA6-A001外源插入片段侧翼序列及其应用[J].大豆科学,2024,43(1):29-34,6.基金项目
吉林省农业科技创新工程项目定向委托(CXGC2022DX009) （CXGC2022DX009）
大豆产业技术体系种子生产岗位专家项目(CARS04-P13). （CARS04-P13）
