吉林医药学院学报2024，Vol.45Issue(2)：97-100,4.
人参皂苷Rg1对小鼠生精改善作用的研究
Study on Improving Effect of Ginsenoside Rg1 on Spermatogenesis in Mice
摘要
Abstract
Objective To study the protective effect of ginsenoside Rg1 on dibutyl phthalate(DBP)-induced spermatogenic injury in mice and to provide a theoretical basis for the development of natural spermatogenic drugs.Methods Thirty C57BL/6 male mice were ran-domly divided into three groups:control group,DBP group(400 mg/kg)and Rg1+DBP group(Rg1 20 mg/kg,DBP 400 mg/kg).After con-tinuous administration for 35 days,the mice were killed,and serum,testis and epididymis were collected.Sperm density,motility and mal-formation rate were detected and calculated,and levels of testosterone(T),follicle-stimulating hormone(FSH)and luteinizing hormone(LH)in serum were detected by enzymelinked immunosorbent assay.The contents of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in mouse testis were determined.Results Compared with the control group,the sperm motility and density in the DBP group were significantly decreased(P<0.01),and the sperm malformation rate was not significantly changed.T and LH in serum were significantly decreased,and FSH in serum was significantly increased(P<0.01).The levels of GSH-Px and SOD in testicular tissue were significantly decreased,and the content of MDA was increased(P<0.01).Compared with the DBP group,sperm motility and density in the DBP+Rg1 group were significantly increased(P<0.01),but the sperm malformation rate was not significantly changed.T and LH in serum were significantly increased,and FSH in serum was significantly decreased(P<0.01).The levels of GSH-Px and SOD in testicular tissue were significantly increased,while the content of MDA was significantly decreased(P<0.01).Conclusion Ginsenoside Rg1 could improve DBP-induced reproductive function injury,and the mechanism may be related to the antioxidant effect of ginsenoside Rg1.关键词
人参皂苷Rg1/生殖功能/邻苯二甲酸二丁酯Key words
ginsenoside Rg1/reproductive function/dibutyl phthalate分类
医药卫生引用本文复制引用
于佳鑫,蔡政国,郭倩梦,吴志俊,李明欣,曲振廷,李环,徐小磊..人参皂苷Rg1对小鼠生精改善作用的研究[J].吉林医药学院学报,2024,45(2):97-100,4.基金项目
大学生创新创业训练计划项目(202210201149,202210201021) （202210201149,202210201021）
吉林省科技厅自然科学基金项目(20220101312JC) （20220101312JC）
吉林省中医药管理局道地药材生精保护作用重点研究室建设项目(2022-Z-72) （2022-Z-72）
吉林省中医药管理局抗衰老中药重点研究室建设项目(2022-Z-73) （2022-Z-73）
