广西植物2024，Vol.44Issue(2)：327-332,6.DOI:10.11931/guihaia.gxzw202210074
马醉木二萜成分及其乙酰胆碱酯酶抑制活性研究
Diterpenoids with acetylcholinesterase inhibitory activity from Pieris japonica
摘要
Abstract
In order to investigate the diterpenoid components from the leaves of Pieris japonica and their acetylcholinesterase(AChE)inhibitory activities,with the aid of thin-layer chromatography color characteristics,silica gel,MCI and semi-preparative high-performance liquid chromatography technology,the target constituents were isolated and purified.The structures of the obtained compounds were identified by analyses of their spectral data(NMR and MS)and comparison of their data with those of reported in the literature.Meanwhile,AChE inhibitory effects of obtained diterpenoids were evaluated for the first time by a Ellman method.The results showed that eight diterpenoid compounds were isolated and identified from the leaves of P.japonica,namely pieriformoside F(1),3-epi-grayanotoxin ⅩⅧ(2),3-epi-grayanotoxin B(3),asebotoxin-X(4),pieriformosin B(5),asebotoxin Ⅲ(6),rhodojaponin Ⅲ(7),and pieriformosin C(8).Among them,Compound 1 was isolated from this plant for the first time,and Compound 8 showed AChE inhibitory activity.In conclusion,the results enriches the diterpenoids and bioactive components of P.japonica,which provides a certain theoretical basis for its further development and utilization.关键词
杜鹃花科/马醉木/二萜/pierisformoside F/乙酰胆碱酯酶抑制活性Key words
Ericaceae/Pieris japonica/diterpenoids/pierisformoside F/acetylcholinesterase inhibitory activities分类
农业科技引用本文复制引用
李慧娟,全伟,罗娥娥,秦徐杰,华燕..马醉木二萜成分及其乙酰胆碱酯酶抑制活性研究[J].广西植物,2024,44(2):327-332,6.基金项目
国家重点研发计划项目(2021YFD1000202) （2021YFD1000202）
国家自然科学基金(31970377) （31970377）
云南省自然科学基金(202001AW070010). （202001AW070010）
