广西植物2024，Vol.44Issue(2)：354-361,8.DOI:10.11931/guihaia.gxzw202303011
甜茶的化学成分及其α-葡萄糖苷酶抑制活性研究
Chemical constituents from Rubus suavissimus and their α-glucosidase inhibitory activities
摘要
Abstract
Rubus suavissimus is mainly distributed in Guilin,Liuzhou,Wuzhou and other regions in Guangxi Zhuang Autonomous Region,so it is called as"Guangxi tiancha"in China.R.suavissimus,together with Siraitia grosvenorii and Stevia rebaudianastevia are praised as three famous sweet plants in Guangxi Zhuang Autonomous Region.Zhuang and Yao people use the leaves of R.suavissimus as a tea to treat diabetes,and it is known as the"divine tea"of Yao medicine.Therefore,R.suavissimus is a combination of sugar,tea and medicine,which has great potential for the development of food and medicine.In order to investigate the secondary metabolites with α-glucosidase inhibitory activity from R.suavissimus,herein,the extraction,separation and purification of secondary metabolites were performed on the leaves of R.suavissimus.The structures of purified compounds were determined based on the data of mass spectrometry(HR-ESI-MS)and nuclear magnetic resonance(1 H NMR and 13C NMR).In addition,the α-glucosidase inhibitory activity of the purified compounds were evaluated by pharmacological methods simultaneously.The results were as follows:(1)Ten compounds were purified and their structures were elucidated as rubusoside(1),kaempferol 3-O-robinobioside(2),gallic acid(3),dihydrodehydroconiferyl alcohol(4),5-methoxydihydrodehydroconiferyl alcohol(5),brevifolincarboxylic acid(6),steviolmonoside(7),steviol(8),16α,17-dihydroxykaurane(9),and quercetin 3-O-β-D-galactopyranoside(10).Among them,compounds 2,4,5 and 9 were isolated from R.suavissimus for the first time.(2)Compounds 2,3,5,6 and 10 showed strong inhibitory activity on α-glucosidase.The IC50 values of compounds 2,3,5,6 and 10 were(0.14±0.03)mg·mL-1,(0.36±0.02)mg·mL-1,(0.44±0.01)mg·mL-1,(0.53±0.04)mg·mL-1 and(0.14±0.03)mg·mL-1 respectively,which were stronger than the positive control acarbose with the IC50 values as(0.69±0.02)mg·mL-1.Thus,compounds 2,3,5,6 and 10,which were isolated from the leaves of R.suavissimus,could be a potential α-glucosidase inhibitors based on their bioactivity results.Compounds with α-glucosidase inhibitory activity from R.suavissimus will provide the basis for development of related hypoglycemic products.关键词
甜茶/α-葡萄糖苷酶/悬钩子属/蔷薇科/糖尿病Key words
Rubus suavissimus/α-glucosidase/Rubus/Rosaceae/diabetes分类
农业科技引用本文复制引用
阳丙媛,何瑞杰,王亚凤,颜小捷,黄永林..甜茶的化学成分及其α-葡萄糖苷酶抑制活性研究[J].广西植物,2024,44(2):354-361,8.基金项目
广西科学院基本科研业务费项目(CQZ-C-1901) （CQZ-C-1901）
广东省重点领域研发计划项目(2020B1111110003). （2020B1111110003）
