临床与病理杂志2023，Vol.43Issue(12)：2063-2070,8.DOI:10.11817/j.issn.2095-6959.2023.230291
组蛋白甲基转移酶NSD3对肾癌细胞增殖、侵袭及迁移的影响
Effect of histone methyltransferase NSD3 on the proliferation,invasion,and migration of renal cancer cells
摘要
Abstract
Objective:The biological role of histone methyltransferase nuclear receptor suppressor of variegation,enhancer of zeste,and trithorax domain-containing 3(NSD3)in renal cancer remains unclear.This study aims to investigate the expression of NSD3 in renal cancer tissues and cells,as well as its effects on proliferation,invasion,and migration of renal cancer cells and the potential molecular mechanisms involved. Methods:The secondary analysis was performed to compare the differential expression of NSD3 mRNA between normal kidney tissues and renal cancer tissues on the gene chip data downloaded from the Gene Expression Omnibus(GEO)database.Real-time polymerase chain reaction(real-time PCR)and Western blotting were performed to detect the expression of NSD3 in normal renal tubular epithelial HK-2 cells and renal cancer 786-O cells,as well as the knockdown efficiency of NSD3 small interfering RNAs(siRNAs)in 786-O cells.The effects of NSD3 downregulation on proliferation,invasion,and migration of 786-O cells were detected by cell counting kit-8(CCK-8)assay,Transwell invasion assay,and Transwell migration assay.The activation of extracellular signal-regulated protein kinases 1/2(ERK1/2)signaling pathway and the expression levels of matrix metallopeptidase(MMP)2 and MMP9 protein were determined by Western blotting assay. Results:Compared with normal kidney tissues and cells,NSD3 expression was increased in renal cancer tissues and cells(both P<0.05).Transfection with NSD3 siRNAs to knockdown NSD3 expression weakened the proliferation,invasion,and migration abilities of 786-O cells(all P<0.05),inhibited the activation of ERK1/2 signaling pathway,and decreased the expression levels of MMP2 and MMP9 protein(all P<0.05). Conclusion:NSD3 may promote cell proliferation,invasion,and migration of renal cancer cells by regulating the ERK1/2 signaling pathway.关键词
组蛋白甲基转移酶/variegation,zeste增强子和trithorax域3的核受体抑制剂/肾癌/增殖/侵袭/迁移Key words
histone methyltransferase/nuclear receptor suppressor of variegation,enhancer of zeste,and trithorax domain-containing 3/renal cancer/proliferation/invasion/migration引用本文复制引用
韩勖,罗凤宝,马旭怡..组蛋白甲基转移酶NSD3对肾癌细胞增殖、侵袭及迁移的影响[J].临床与病理杂志,2023,43(12):2063-2070,8.基金项目
江苏省双创博士基金(JSSCBS20211591).This work was supported by the fund of Doctor in Innovation and Entrepreneurship of Jiangsu Province,China(JSSCBS20211591). （JSSCBS20211591）
