广西植物2024，Vol.44Issue(2)：396-404,9.DOI:10.11931/guihaia.gxzw202212068
黔产剑叶凤尾蕨的化学成分研究
Chemical constituents of Pteris ensiformis from Guizhou
摘要
Abstract
To study the chemical constituents of Pteris ensiformis,silica gel,gel,MCI,C18 and other column chromatography were used for separation and purification,and their structures were identified by 1H-NMR,13C-NMR,MS,IR and othe spectral data;the anti-tumor and anti-coagulation activities of some monomers were screened by MTS,APTT,PT and TT.The results were as follows:(1)A total of 15 compounds were isolated from P.ensiformis,the compounds were 2-hydroxy-acetylpyrrole(1),N-(3-carboxypropyl)-2-acetylpyrrole(2),3-hydroxy-2-methylpyridine(3),N-methylhydroxylamine(4),pterosin S 13-O-glucoside(5),obtupterosin C(6),ent-11α-hydroxy-15-oxokauran-19-oic acid(7),ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid(8),β-sitosterol(9),ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid-O-glucopyranoside(10),5,5′-dibutoxy-2,2′-bifuran(11),5,5′-di(2-ethyl-hexyloxy)-2,2′-bifuran(12),(-)-loliolide(13),succinic acid(14),fumaric acid(15).Compound 1 is a new natural product of pyrrole alkaloids.Compounds 1-7,10-15 were isolated from P.ensiformis for the first time,and compounds 1,3,4 were isolated from Pteris for the first time.(2)The results of activity test showed that compounds 1,2,3,5,6 and 10 inhibited the growth of tumor cells HL-60,A549,SMMC-7721,MDA-MB-231 and SW480 in vitro at a concentration of 40 μmol·L-1,At the concentration of 2.0 mmol·L-1,compounds 1,2,3 and 6 shortened APTT and compounds 1,5 and 6 prolonged PT.The study enriches the chemical constituents of P.ensiformis from Guizhou and provides a material basis for the development of anti-tumor drugs.关键词
剑叶凤尾蕨/化学成分/结构鉴定/抗肿瘤活性/抗凝血活性Key words
Pteris ensiformis/chemical constituent/structural identification/anti-tumor activity/anticoagulant activity分类
农业科技引用本文复制引用
肖丽,申琳燕,张敬杰,何康,叶江海,赵臣亮,张奇龙,邹娟..黔产剑叶凤尾蕨的化学成分研究[J].广西植物,2024,44(2):396-404,9.基金项目
国家自然科学基金(81660723) （81660723）
贵州省科技计划项目(黔科合平台人才(2017)5618) （黔科合平台人才(2017）
贵州省中医药管理局项目(QZYY-2015-125). （QZYY-2015-125）
