摘要
Abstract
Objective:To investigate the correlation between the expression of fibrinogen-like protein 1(FGL1)and the prognosis of patients with oral squamous cell carcinoma,and to analyze the role of FGL1 in the proliferation,migration,invasion,and epithelial-mesenchymal transformation(EMT)of oral squamous cell carcinoma cells.
Methods:A total of 50 pairs of oral squamous cell carcinoma tissues(oral squamous cell carcinoma group)and corresponding adjacent tissues(adjacent group)were collected.Immunohistochemistry(IHC)staining was performed to detect the percentage of FGL1-positive staining cells.According to the IHC score,the patients were divided into a FGL1 low-expression group and a FGL1 high-expression group.Kaplan-Meier method and Log-rank test were used to analyze the effect of FGL1 expression on the prognosis of patients.The oral squamous cell carcinoma cell line HSC-4 was divided into a small interfering RNA(siRNA)negative control group(si-Con group),a siRNA FGL1(si-FGL1)group,and a co-transfected si-FGL1+β-catenin group.Cell counting kit-8(CCK-8),colony formation,scratch,and Transwell assay were conducted to detect cell growth,migration,and invasion of the HSC-4 cells.Western blotting was performed to determine the expression levels of EMT markers(E-cadherin,N-cadherin,and nuclear β-catenin)in HSC-4 cells.The dual-luciferase reporter gene assay was used to detect the effect of FGL1 on the activation of β-catenin signaling(TOP/FOP ratio).
Results:Compared with the adjacent group,the IHC score of FGL1 protein in the oral squamous cell carcinoma group was significantly increased(P<0.01).Patients in the FGL1 high-expression group had a worse prognosis than those in the FGL1 low-expression group(P<0.01).Compared with the si-Con group,HSC-4 cell proliferation,migration,invasion,and levels of N-cadherin and nuclear β-catenin were decreased,while E-cadherin expression was increased in the si-FGL1 group(all P<0.01).Compared with the si-Con group,the TOP/FOP ratio of HSC-4 cells in the si-FGL1 group was significantly decreased(P<0.01).Compared with the si-FGL1 group,HSC-4 cell proliferation,migration,invasion,and levels of N-cadherin and nuclear β-catenin were increased,while E-cadherin expression was decreased in the si-FGL1+β-catenin group(all P<0.01).
Conclusion:FGL1 is highly expressed in oral squamous cell carcinoma tissues,and its high expression is associated with poor prognosis in patients with oral squamous cell carcinoma.FGL1 can regulates the proliferation,migration,invasion,and EMT of oral squamous cell carcinoma cells by modulating the expression of nuclear β-catenin in HSC-4 cells.关键词
纤维蛋白原样蛋白1/口腔鳞状细胞癌/侵袭/迁移/上皮间质转化/β-连环素Key words
fibrinogen-like protein 1/oral squamous cell carcinoma/invasion/migration/epithelial mesenchymal transformation/β-catenin
