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甲型流感病毒H1N1型灭活病毒标准物质研究

许丽乐丽欢杨雪王炤坤李兰英闻艳丽陶晴胡轶红刘刚

计量学报2024，Vol.45Issue(2)：294-299,6.

计量学报2024，Vol.45Issue(2)：294-299,6.DOI:10.3969/j.issn.1000-1158.2024.02.21

甲型流感病毒H1N1型灭活病毒标准物质研究

Research of a Reference Material of Inactivated Influenza A(H1N1)Virus

许丽 ¹乐丽欢 ²杨雪 ¹王炤坤 ¹李兰英 ¹闻艳丽 ¹陶晴 ¹胡轶红 ²刘刚¹

作者信息

1. 上海市计量测试技术研究院国家市场监管重点实验室(生物分析计量溯源),上海 201203
2. 中国科学院上海免疫与感染研究所公共技术服务中心病原发现与大数据平台,上海 200031
折叠

摘要

Abstract

A reference material(RM)of inactivated influenza A(H1N1)virus was developed.The RM was made of artificially cultured natural virus as raw materials.After chemical inactivation and verification of the inactivation effect,the copy number concentration was determined by digital PCR(dPCR)in different laboratories.The homogeneity,stability and uncertainty of the RM were evaluated in detail.As the results demonstrated,the RM can be stably stored for more than 5 months under-80～-70 ℃.The final certified value of reference materials was(1 384.5±249.3)copies/μL(k=2).The RM can be used for the metrological traceability and quality control in the whole process of influenza A virus H1N1 detection and help to improve the accuracy and reliability of related nucleic acid test results.

关键词

生物计量学/甲型流感病毒/H1N1/灭活病毒/标准物质/数字PCR

Key words

biometrology/influenza A virus,H1N1,inactivated virus,reference material,digital PCR

分类

通用工业技术

引用本文复制引用

许丽,乐丽欢,杨雪,王炤坤,李兰英,闻艳丽,陶晴,胡轶红,刘刚..甲型流感病毒H1N1型灭活病毒标准物质研究[J].计量学报,2024,45(2):294-299,6.

基金项目

国家自然科学基金(22074093) （22074093）

国家市场监督管理总局技术保障专项(2022YJ10) （2022YJ10）

计量学报

OA北大核心CSTPCD

ISSN：1000-1158

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