海洋科学2023，Vol.47Issue(12)：30-39,10.DOI:10.11759/hykx20230319001
大菱鲆幼鱼视网膜结构及不同光谱下视蛋白基因表达特征的研究
Development of retinal structures and opsin gene expression under different spectra in juvenile turbot
摘要
Abstract
Turbot(Scophthalmus maximus)is an economically important cultured fish.Its retinal structure and light-sensing ability vary during its lifespan as it adapts to the change in its niche from the planktonic to the benthic region.In this study,we studied juvenile turbot at different developmental stages and assessed the development of retinal structures and expression of opsin genes in response to different light spectra.We observed that the outer thickness of the retinal nucleus increased as the turbot grew,whereas the thickness of rods and cones remained con-stant.Additionally,the inner nuclear layer and layer of ganglion cells shrank.The expression ratio of rh1 to total opsin genes increased gradually from 57.35％in two-month-old turbot to 77.19％in nine-month-old turbot.The ratio of the expression of cone opsin genes to total opsin genes decreased,particularly for lws,whose expression proportion changed from 4.49％at two months to 0.13％at nine months.Moreover,strong correlations were de-tected between opsin expression patterns in response to different light spectra.The expression of rh2b1,sws2,and sws1 decreased significantly in seven-month-old turbot under red and yellow light(P＜0.05),whereas the expression of rh2b1 increased under blue and green light.The expression of sws2 increased only under the green light.The rh2 family of opsins dominated the expression among opsins under yellow,green,and blue light and under full spec-trum light.The only dominant opsin gene after treatment with red light was rh1.Our results revealed a change in turbot opsins upon varying the light spectra,probably in coordination with its life span at different depths.There-fore,our study may provide essential genomic resources for future industrial applications in the turbot industry.关键词
大菱鲆(Scophthalmus maximus)/视网膜结构/视蛋白/光谱适应性Key words
Scophthalmus maximus/retina/opsin/spectrum adaptation分类
生物科学引用本文复制引用
刘峰,吴乐乐,王雨浓,齐婷,徐世宏,李军,李贤..大菱鲆幼鱼视网膜结构及不同光谱下视蛋白基因表达特征的研究[J].海洋科学,2023,47(12):30-39,10.基金项目
国家自然科学基金资助项11(41976122,32273104) （41976122,32273104）
现代农业产业技术体系国家海水鱼体系(CARS47)[Natural Science Foundation of China,Nos,41976122,32273104 （CARS47）
Modern Agricultural Industry Technology System National Marine Fish System,No.CARS 47] （）
