脱矿牙本质基质和脱细胞牙本质基质成骨效果的对比研究OA北大核心CSTPCD
Comparison of the osteogenic effects of demineralized dentin matrix and acellular dentin matrix
目的 比较骨缺损区植入脱矿牙本质基质和脱细胞牙本质基质的成骨效果.方法 制备脱矿牙本质基质和脱细胞牙本质基质.将24只SPF级SD雄性大鼠随机分为脱矿组(A组)、脱细胞组(B组)、Bio-Oss骨粉组(C组)、空白组(D组),每组6只大鼠,在麻醉条件下制备双侧股骨骨缺损.A、B、C组大鼠分别在骨缺损区植入脱矿牙本质基质、脱细胞牙本质基质、Bio-Oss骨粉,D组大鼠不植入任何材料.术后4周和8周,每组各随机处死3只大鼠.大体观察骨缺损区愈合情况,血清学检测成骨指标骨形态发生蛋白(BMP)-2及碱性磷酸酶(ALP)浓度,影像学观察骨缺损区高密度灰色区(代表骨愈合)分布情况,组织形态学观察新骨形成情况,计算新骨形成率.结果 术后4周和8周,大体观察见A组成骨能力较其他组活跃,血清学检测A组BMP-2及ALP浓度均高于其他组,差异有统计学意义(P<0.05).8周时,影像学观察可见A组骨缺损区高密度灰色区分布均匀,组织形态学观察见A组排列规则的骨基质.A组4、8周时的新骨形成率分别为28.51%±0.55%、32.57%±2.28%,均高于其他组,差异有统计学意义(P<0.05).结论 脱矿牙本质基质比脱细胞牙本质基质具有更好的成骨潜能.
Objective This study aims to compare the osteogenic effects of implanting demineralized dentin matrix and acellular dentin matrix in bone defect areas.Methods Demineralized dentin matrix and acellular dentin matrix were prepared.Twenty-four male SPF-grade SD rats were randomly divided into four groups:demineralized group(group A),acelluar group(group B),Bio-Oss bone powder group(group C),and blank control group(group D),with six rats in each group.All rats were subjected to general anesthesia to prepare bilateral femoral bone defects.Rats in groups A,B,and C were implanted with demineralized dentin matrix,acellular dentin matrix,and Bio-Oss bone powder at the bone defect area,respectively,while rats in group D were not implanted with any material.At 4 and 8 weeks after surgery,three rats were randomly executed in each group.The healing of the bone defect area was ana-lyzed through gross observation.The concentrations of osteogenic indicators bone morphogenetic protein-2(BMP-2)and alkaline phosphatase(ALP)were detected by serology.The distribution of high-density gray area(representing bone healing)in the bone defect area was observed by X-ray examination,and the formation of new bone was observed by his-tomorphology.The rate of new bone formation was calculated.Results At 4 and 8 weeks,the bone formation ability of group A was more active than that of the other groups;the concentrations of BMP-2 and ALP in group A were higher than those in the other groups,and the differences were statistically significant(P<0.05).At 8 weeks,the imaging obser-vation showed that the high-density gray area in the bone defect of group A was evenly distributed.The histomorphologi-cal observation showed the regular arrangement of bone matrix in group A.The rate of new bone formation in group A was 28.51%±0.55%at 4 weeks and 32.57%±2.28%at 8 weeks,both of which were significantly higher than those in the other groups(P<0.05).Conclusion Demineralized dentin matrix has better osteogenic potential than acellular dentin matrix.
达尔亚·俄尼木拜;张迪;古丽努尔·阿吾提
新疆医科大学第一附属医院/附属口腔医(学)院牙周病科,乌鲁木齐 830011新疆医科大学第一附属医院/附属口腔医(学)院牙周病科,乌鲁木齐 830011||新疆维吾尔自治区口腔医学研究所,乌鲁木齐 830011
生物工程
脱矿牙本质基质脱细胞牙本质基质骨修复骨移植材料
demineralized dentin matrixacellular dentin matrixbone repairbone graft material
《华西口腔医学杂志》 2024 (001)
28-36 / 9
Western Clinical Research Foundation of Stomatology of Chinese Academy of Stomatology(CSA-W2020-11) [基金项目]中华口腔医学会西部口腔医学临床科研基金(CSA-W-2020-11)
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