华西口腔医学杂志2024，Vol.42Issue(1)：28-36,9.DOI:10.7518/hxkq.2023.2023161
脱矿牙本质基质和脱细胞牙本质基质成骨效果的对比研究
Comparison of the osteogenic effects of demineralized dentin matrix and acellular dentin matrix
摘要
Abstract
Objective This study aims to compare the osteogenic effects of implanting demineralized dentin matrix and acellular dentin matrix in bone defect areas.Methods Demineralized dentin matrix and acellular dentin matrix were prepared.Twenty-four male SPF-grade SD rats were randomly divided into four groups:demineralized group(group A),acelluar group(group B),Bio-Oss bone powder group(group C),and blank control group(group D),with six rats in each group.All rats were subjected to general anesthesia to prepare bilateral femoral bone defects.Rats in groups A,B,and C were implanted with demineralized dentin matrix,acellular dentin matrix,and Bio-Oss bone powder at the bone defect area,respectively,while rats in group D were not implanted with any material.At 4 and 8 weeks after surgery,three rats were randomly executed in each group.The healing of the bone defect area was ana-lyzed through gross observation.The concentrations of osteogenic indicators bone morphogenetic protein-2(BMP-2)and alkaline phosphatase(ALP)were detected by serology.The distribution of high-density gray area(representing bone healing)in the bone defect area was observed by X-ray examination,and the formation of new bone was observed by his-tomorphology.The rate of new bone formation was calculated.Results At 4 and 8 weeks,the bone formation ability of group A was more active than that of the other groups;the concentrations of BMP-2 and ALP in group A were higher than those in the other groups,and the differences were statistically significant(P<0.05).At 8 weeks,the imaging obser-vation showed that the high-density gray area in the bone defect of group A was evenly distributed.The histomorphologi-cal observation showed the regular arrangement of bone matrix in group A.The rate of new bone formation in group A was 28.51%±0.55%at 4 weeks and 32.57%±2.28%at 8 weeks,both of which were significantly higher than those in the other groups(P<0.05).Conclusion Demineralized dentin matrix has better osteogenic potential than acellular dentin matrix.关键词
脱矿牙本质基质/脱细胞牙本质基质/骨修复/骨移植材料Key words
demineralized dentin matrix/acellular dentin matrix/bone repair/bone graft material分类
生物科学引用本文复制引用
达尔亚·俄尼木拜,张迪,古丽努尔·阿吾提..脱矿牙本质基质和脱细胞牙本质基质成骨效果的对比研究[J].华西口腔医学杂志,2024,42(1):28-36,9.基金项目
Western Clinical Research Foundation of Stomatology of Chinese Academy of Stomatology(CSA-W2020-11) [基金项目]中华口腔医学会西部口腔医学临床科研基金(CSA-W-2020-11) （CSA-W2020-11）
