华西口腔医学杂志2024，Vol.42Issue(1)：46-55,10.DOI:10.7518/hxkq.2024.2023237
miR-362-3p调控垂体肿瘤转化基因1抑制口腔鳞状细胞癌侵袭及增殖的研究
miR-362-3p inhibited the invasion and metastasis of oral squamous cell carcinoma cells by targeting the regula-tion of pituitary tumor-transforming gene 1
摘要
Abstract
Objective This study aimed to explore the effect of pituitary tumor-transforming gene 1(PTT-G1)on the invasion and proliferation of oral squamous cell carcinoma(OSCC)cell lines under the action of miR-362-3p.Methods The bioinformatics online database was used to query the expression of PTTG1 in head and neck squamous cell carcinoma(HNSCC).The expression of PTTG1 in the Cal-27,HN-30,and HOK cell lines was detected by Western blot.A wound-healing assay was used to determine the effect of PTTG1 on the migration ability of the OSCC cells.The Transwell assay was used to examine the changes in cell-invasion ability.5-ethynyl-2'-deoxyuridine(EdU)cell-proliferation assay was used to detect changes in cell-proliferation ability.Bioinformatics approach predicted the upstream miRNA of PTTG1.The targeting relationship between miR-362-3p and PTTG1 was examined by the dual luciferase assay,and quantitative real-time polymerase chain reaction(qRT-PCR)was used to determine the expression of miRNA in OSCC tissues.Results The ENCORI database showed that PTTG1 expression was up-regulated in OSCC tissues.Western blot confirmed that PTTG1 expression was up-regulated in Cal-27 and HN-30 cells than HOK cells.PTTG1 knockout can inhibit the migration,invasion,and prolif-eration of Cal-27 and HN-30 cells(P<0.05).Bioinformatics prediction websites predicted that the upstream miRNA of PTTG1 was miR-362-3p,and PTTG1 can bind to miR-362-3p.Results of qRT-PCR showed that miR-362-3p expression was downregulated in OSCC tissues compared with normal tissue(P<0.05).Transwell and EdU experiments confirmed that miR-362-3p knockdown can promote the invasion and proliferation of Cal-27 and HN-30 after PTTG1 knockdown.Conclusion miR-362-3p can inhibit the invasion and proliferation of Cal-27 and HN-30 cells by targeting PTTG1.关键词
口腔鳞状细胞癌/垂体肿瘤转化基因1/微小RNA/侵袭/增殖Key words
oral squamous cell carcinoma/pituitary tumor-transforming gene 1/microRNA/invasion/prolif-eration分类
生物科学引用本文复制引用
丁啸,陈嘉雯,曲鹏宇,孙晨雨,李洪利,胡温庭,范欣..miR-362-3p调控垂体肿瘤转化基因1抑制口腔鳞状细胞癌侵袭及增殖的研究[J].华西口腔医学杂志,2024,42(1):46-55,10.基金项目
Natural Science Foundation Project of Shandong Province(ZR202110190030) （ZR202110190030）
Fundamental Research Program Funding of Affiliated Hospital of Weifang Medical University(2021wffyzzjj06) （2021wffyzzjj06）
Weifang Science and Technolo-gy Development Plan Project(2022YX029) [基金项目]山东省自然科学基金项目(ZR202110190030) （2022YX029）
潍坊医学院附属医院种子基金项目(2021wffyzzjj06) （2021wffyzzjj06）
潍坊市科技发展计划项目(2022YX029) （2022YX029）
