吉首大学学报（自然科学版）2024，Vol.45Issue(1)：66-76,11.DOI:10.13438/j.cnki.jdzk.2024.01.012
毛茛铁线莲叶绿体全基因组解析及系统发育分析
Complete Chloroplast Genome of Clematis ranunculoides:Characterization and Phylogeny Analysis
摘要
Abstract
To reveal the chloroplast genome characteristics of Clematis ranunculoides and its phylogenetic relationships,the present study adopted the Illumina platform to acquire high-throughput sequences,which were used for assemble,annotate,and characterize the complete chloroplast genome of C.ranun-culoides.And then molecular phylogenetic trees were constructed with maximum parsimony(MP),max-imum likelihood(ML)and Bayesian(BI)methods.The result shows that the total length of the chloro-plast genome of C.ranunculoides is 159 741 bp,which encodes 112 genes,including 79 protein-coding genes,29 tRNA genes and 4 rRNA genes;there are 84 SSRs in the chloroplast genome of C.ranuncu-loides,and 57 of them are mono-nucleotide.Analysis of codon usage bias reveales that leucine is the most frequently utilized amino acid in the chloroplast genome.The topological structures are basically identical among MP,ML,and BI trees.Derived from the phylogenetic trees,Clematis is a monophyletic group,closely related to Anemone.And C.ranunculoides is closest to C.montana,which supportes the tradi-tional classification results.关键词
毛茛铁线莲/叶绿体基因组/简单重复序列/系统发育/毛茛科Key words
Clematis ranunculoides/chloroplast genome/simple sequence repeats/phylogeny/Ranuncu-laceae分类
农业科技引用本文复制引用
王若澜,张德全..毛茛铁线莲叶绿体全基因组解析及系统发育分析[J].吉首大学学报（自然科学版）,2024,45(1):66-76,11.基金项目
国家自然科学基金资助项目(32060091) （32060091）
云南省中青年学术和技术带头人后备人才项目(2105AC160063) （2105AC160063）
