生物灾害科学2024，Vol.47Issue(1)：21-27,7.DOI:10.3969/j.issn.2095-3704.2024.01.04
白莲缘枯病病原菌的分离鉴定与防治药剂筛选
Identification of leaf margin blight pathogen on lotus and its fungicide screening
摘要
Abstract
[Objective]In recent years,leaf margin blight of lotus has occurred frequently in Jiangxi Province,causing a serious negative impact on the yield and quality of lotus seeds.This study was conducted to identify the pathogen causing leaf margin blight of lotus and screen fungicides for the disease prevention and control.[Method]This study identified the pathogen by using morphology,molecular biology and Koch's rule,combined with the morphology and sequence analysis of ITS,TEF1-α and β-tubulin.[Result]It was determined that the isolated strain was Nelumbo parvum.The mycelia growth rate method was used to determine the indoor toxicity of six fungicides to the pathogen.And the chemical agents suitable for the prevention and control of the disease were screened.Among six fungicides,45%prochloraz,50%carbendazim wettable powder and 40%difenoconazole had the most obvious inhibitory effects,and the EC50 were 0.010,0.050 and 0.025 μg/mL,respectively.[Conclusion]The three fungicides of 45%prochloraz,50%carbendazim wettable powder and 40%difenoconazole had better bactericidal effect on Nelumbo parvum.关键词
白莲/缘枯病/病原菌鉴定/小新壳梭孢/药剂筛选Key words
lotus/leaf margin blight/pathogen identification/Nelumbo parvum/fungicide screening分类
农业科技引用本文复制引用
朱玲,付钰铭,陈兰兰,舒梦霜,张潇月,郑兴汶,崔汝强..白莲缘枯病病原菌的分离鉴定与防治药剂筛选[J].生物灾害科学,2024,47(1):21-27,7.基金项目
江西省抚州市青年科技领军人才项目(2020ED73) （2020ED73）
