生物灾害科学2024，Vol.47Issue(1)：42-49,8.DOI:10.3969/j.issn.2095-3704.2024.01.07
巨大芽孢杆菌TA-4的筛选鉴定及对番茄防御酶活性的影响
Screening and identification of Bacillus megaterium TA-4 and its effect on the activities of tomato defense enzymes
摘要
Abstract
[Objective]This study was conducted to screen biological control bacteria with indoleacetic acid(IAA)producing ability and explore the potential of growth-promoting ability.[Method]In the present study,the plate-counting and Salkowski colorimetric methods were used to screen growth-promoting bacteria with IAA producing ability from the fumigated tomato soil.After identified the bacterium with the best IAA production,pot experiments were used to determine the tomato growth promotion effect of the strain and its effects on the activities of defense enzymes.[Result]The results showed that the largest amount of IAA produced by TA-4 was 39.0 µg/mL.TA-4 was identified as Bacillus megaterium according to the results of colony morphology,physiological and biochemical and molecular biological technology.In the pot experiments,the effects of TA-4 bio-organic fertilizer on the activities of defense enzymes of tomato plants including phenylalanine ammonia lyase(PAL),polyphenol oxidase(PPO)and peroxidase(POD)were explored.The results also showed that,at 30 days after treatment,2%TA-4 bio-organic fertilizer notably enhanced tomato growth.Compared with the non-treated control,the application of TA-4 bio-organic fertilizer significantly increased the plant height,stem diameter,fresh weight and dry weight of tomato plants by 58.7%,27.3%,53.2%and 70.6%,respectively.Meanwhile,the activities of defense enzymes including PAL,PPO and POD in tomato plants significantly increased compared to that of the non-treated control,which effectively enhanced the plant growth and induced host resistance in the tomato against adverse environments.[Conclusion]The results of this study determined the effects of TA-4 on the plant growth and activities of tomato defense enzymes,providing a theoretical basis for the research and development of new bio-organic fertilizers.关键词
巨大芽孢杆菌/生物有机肥/防御酶Key words
Bacillus megaterium/bio-organic fertilizer/defensive enzyme分类
农业科技引用本文复制引用
姚起,宋国栋,乔康..巨大芽孢杆菌TA-4的筛选鉴定及对番茄防御酶活性的影响[J].生物灾害科学,2024,47(1):42-49,8.基金项目
山东省自然科学基金项目(ZR2021MC065)和山东省科技型中小企业创新能力提升工程项目(2023TSGC0613) （ZR2021MC065）
