经济动物学报2023，Vol.27Issue(4)：268-274,7.DOI:10.13326/j.jea.2023.1663
基于LC-MS/MS技术对CpA感染鸭十二指肠代谢组学分析
Analysis of Duodenal Metabolomics of CpA Infected Ducklings Based on LC-MS/MS Technology
摘要
Abstract
To investigate the changes of duodenal metabolites of ducks infected with Clostridium perfringens alpha toxin(CpA),and to search for potential biomarkers and metabolic pathways related to the pathogenesis,twelve 37-day-old ducks were randomly divided into control group and CpA infected group with 6 ducks in each group.Liquid chromatoc-tandem mass spectrometry(LC-MS/MS)was used to analyze the non-targeted metabolomics of duodenum in normal group and 90 h CpA infected ducks.The results showed that in partial least squares discriminant analysis(PLS-DA)model,the score maps of control group and CpA infection group showed significant differences,and some metabolites in the CpA infection group had significant changes.After 90 h of CpA infection,29 different metabolites such as arachidonic acid,3-hydroxybutyric acid,2,3-dihydroxybenzoic acid and adenosine diphosphate were identified,and 25 signaling pathways were mainly involved in unsaturated fatty acid biosynthesis,β-alanine metabolism,arachidonic acid metabolism,sulfur metabolism,pyruvate metabolism and purine metabolism.There were differences in lipid metabolism,amino acid metabolism and carbon metabolism of duodenum between CpA infected group and control group.The results of this study could provide a new basis for the study of duodenal injury mechanism caused by CpA infection in ducks.关键词
A型产气荚膜梭菌/鸭/十二指肠/液相色谱-串联质谱(LC-MS/MS)/代谢组学Key words
Clostridium perfringens alpha toxin/ducklings/duodenum/liquid chromatography-tandem mass spectrometry(LC-MS/MS)/metabolomics分类
农业科技引用本文复制引用
王娜,张天天,程振涛,文明,王开功,周碧君..基于LC-MS/MS技术对CpA感染鸭十二指肠代谢组学分析[J].经济动物学报,2023,27(4):268-274,7.基金项目
贵州省农业攻关项目[黔科合NY(2015)3009-1],贵州省研究生工作站计划项目(GZZ2017002),贵州省科技平台及人才团队计划项目[黔科合平台人才(2018)5253] （2015）
