环境与职业医学2024，Vol.41Issue(2)：119-124,132,7.DOI:10.11836/JEOM23198
长期睡眠期噪声暴露对小鼠认知功能的影响及生物钟相关机制
Effects of long-term noise exposure during sleep on cognitive function and biological clock-re-lated mechanisms in mice
摘要
Abstract
[Background]Environmental noise pollution is serious,and there are few studies on the effects of long-term noise exposure during sleep on cognitive function and possible biological clock mechanism. [Objective]To explore the cognitive impairment induced by noise exposure during sleep in mice and possible biological clock mechanism,and to provide a theoretical basis for the protection against noise exposure. [Methods]Twenty male C57BL/6J mice were randomly divided into a control group and a noise-exposed group,10 mice in each group.The noise-exposed group was exposed to sleep-period noise using a noise generator for 12 h(08:00-20:00)per day for a total of 30 d.The calibrated noise intensity was set at 90 dB.No intervention was imposed on the control group.At the end of the noise exposure,cognitive function of mice was examined using the new object recognition experiment and the open field test,and the hippocampal tissue damage of mice were evaluated by Nissl staining,ionized calcium binding adaptor molecule 1(Iba1)immunofluorescence staining,and real-time fluores-cence quantitative PCR for inflammatory factors and biological clock genes.Oxidative stress indicators in the hippocampus of mice were also detected by assay kit. [Results]After noise exposure during sleep period,the results of new object recognition experiment showed that the discrimination index of mice in the noise-exposed group was 0.06±0.04,which was significantly lower than that of the control group(0.65±0.13)(P<0.05).The results of open field test showed that the central activity distance of the noise-exposed group was(242.20±176.10)mm,which was significantly lower than that of the control group,(1548.00±790.30)mm(P<0.05),and the central activity time of the noise-exposed group was(0.87±0.64)s,which was significantly lower than that of the control group,(6.00±2.86)s(P<0.05).The Nissl staining results showed that compared with the control group,neurons in the hippocampus of the noise-exposed mice were shrunken,deeply stained,disorganized,and loosely connected.The immunofluorescence results showed that microglia in the hippocampus of the noise-exposed mice were activated and the expression of Iba1 was significantly increased compared with those of the control group(P<0.05).The real-time PCR results of showed that the mRNA levels of the biological clock genes Clock,Per2,and Rev-erbα were significantly increased compared with those of the control group(P<0.05),and the mRNA level of Per1 was significantly decreased compared with that of the control group(P<0.05);and the mRNA levels of IL-18,IL-6,iNOS,and NLRP3 in the hippocampal tissues of mice were significantly increased compared with those of the control group(P<0.05).The results of oxidative stress evaluation showed that compared with the control group,reduced glutathione content was significantly reduced in the noise-exposed group(P<0.001). [Conclusion]Noise exposure during sleep period can lead to the destabilization of biological clock genes in hippocampal tissues and trigger hippocampal neuroinflammation,which can lead to the activation of microglia and cause cognitive impairment in mice.关键词
睡眠期噪声暴露/神经炎症/认知损伤/生物钟Key words
sleep phase noise exposure/neuroinflammation/cognitive impairment/biological clock分类
医药卫生引用本文复制引用
付一鸣,张鑫垚,佘晓俊,朱英文,杨红莲,杲修杰,付波,崔博..长期睡眠期噪声暴露对小鼠认知功能的影响及生物钟相关机制[J].环境与职业医学,2024,41(2):119-124,132,7.基金项目
国家自然科学基金面上项目(81673136) This study was funded. （81673136）
