环境与职业医学2024，Vol.41Issue(2)：125-132,8.DOI:10.11836/JEOM23222
红景天苷对PM2.5暴露小鼠肠道微生物的调节作用
Moderating effect of salidroside on intestinal microbiota in mice exposed to PM2.5
摘要
Abstract
[Background]Salidroside(SAL)has a protective effect on multiple organ systems.Exposure to fine particulate matter(PM2.5)in the atmosphere may lead to disruptions in gut microbiota and impact intestinal health.The regulatory effect of SAL on the gut microbiota of mice exposed to PM2.5 requires further investigation. [Objective]To evaluate gut microbiota disruption in mice after being exposed to PM2.5 and the potential effect of SAL. [Methods]Forty male C57BL/6 mice,aged 6 to 8 weeks,were randomly divided into four groups:a control group,an SAL group,a PM2.5 group,and an SAL+PM2.5 group,each containing 10 mice.In the SAL group and the SAL+PM2.5 group,the mice were administered SAL(60 mg·kg-1)by gavage,while in the control group and the PM2.5 group,sterile saline(10 mL·kg-1)was administered by gavage.In the PM2.5 group and the SAL+PM2.5 group,PM2.5 suspension(8 mg·kg-1)was intratracheally instilled,and in the control group and SAL group,sterile saline(1.5 mL·kg-1)was intratracheally administered.Each experiment cycle spanned 2 d,with a total of 10 cycles conducted over 20 d.Histopathological changes in the ileum tissue of the mice were observed after HE staining.Colon contents were collected for gut microbiota sequencing and short-chain fatty acids(SCFAs)measurements. [Results]The PM2.5 group showed infiltration of inflammatory cells in the ileum tissue,while the SAL+PM2.5 group exhibited only a small amount of inflammatory cell infiltration.Compared to the control group,the PM2.5 group showed decreased Shannon index(P<0.05)and increased Simpson index(P<0.05),indicating that the diversity of gut microbiota in this group was decreased;the SAL+PM2.5 group showed increased Shannon index compared to the PM2.5 group(P<0.05)and decreased Simpson index(P<0.05),indicating that the di-versity of gut microbiota in mice intervened with SAL was increased.The principal coordinates analysis(PCoA)revealed a significant sep-aration between the PM2.5 group and the control group,while the separation trend was less evident among the control group,the SAL group,and the SAL+PM2.5 group.The unweighted pair-group method with arithmetic means(UPGMA)clustering tree results showed that the control group and the SAL group clustered together first,followed by clustering with the SAL+PM2.5 group,and finally,the three groups clustered with the PM2.5 group.The PCoA and UPGMA clustering results indicated that the uniformity and similarity of the microbiota in the PM2.5 group were significantly decreased.Compared to the control group,the PM2.5 group showed decreased abundance of phylum Bacteroidetes and Candidatus_Saccharimonas(P<0.05)and increased abundance of phylum Proteobacteria,genus Escherichia,genus Bacteroides,genus Prevotella,genus Enterococcus,and genus Proteus(P<0.05).Compared to the PM2.5 group,the SAL+PM2.5 group showed decreased abundance of phylum Proteobacteria,phylum Actinobacteria,genus Prevotella,and genus Proteus(P<0.05),and in-creased abundance of Candidatus_Saccharimonas(P<0.05).The PM2.5 group showed reduced levels of propionic acid,valeric acid,and hexanoic acid compared to the control group(P<0.05),while the SAL+PM2.5 group showed increased levels of propionic acid,isobutyric acid,butyric acid,valeric acid,and hexanoic acid compared to the PM2.5 group(P<0.05). [Conclusion]Exposure to PM2.5 can cause pathological alterations,microbial dysbiosis,and disturbing production of SCFAs in intestinal tissue in mice.However,SAL can provide a certain degree of protective effect against these changes.关键词
PM2.5/红景天苷/肠道微生物/短链脂肪酸/肺-肠轴Key words
PM2.5/salidroside/intestinal microbiota/short-chain fatty acids/lung-gut axis分类
医药卫生引用本文复制引用
李思琪,栾孟孝,韩晓琳,谭金峰,于丽,徐东群,王秦,李晓红,李万伟,刘晨,徐伟红,吴文博,周睿熙,张丽敏,宋超,刘玉梅,谭凤娇..红景天苷对PM2.5暴露小鼠肠道微生物的调节作用[J].环境与职业医学,2024,41(2):125-132,8.基金项目
山东省自然基金项目(ZR2020MH336,ZR2015HL099) （ZR2020MH336,ZR2015HL099）
山东省中医药科技项目(2020M077) （2020M077）
潍坊市科学技术发展计划项目(2022GX010,2022GX015) （2022GX010,2022GX015）
潍坊医学院研究生科研创新基金项目(2023YJSCX010) This study was funded. （2023YJSCX010）
