迷迭香酸-锌配合物的抗氧化和抗菌性研究OA北大核心CSTPCD

Objective:To investigate the structure,antioxidant,antimicrobial and biocompatibility of rosmarinic acid(RA)and zinc(Zn)complexes.Methods:The structures of zinc complexes with rosmarinic acid(RA-Zn)were analyzed by scanning electron microscopy,infrared and ultraviolet-visible spectroscopy.The effects of RA and RA-Zn on the proliferative activity of osteoblasts were compared by cell proliferation assay.The antioxidant properties were analyzed by ferric ion reducing antioxidant power assay and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)ammonium salt(ABTS)free radical scavenging assay.The inhibitory activities of RA and RA-Zn against Straphylococcus aureus were compared by the minimum inhibitory concentration assay.Results:The complex(RA-Zn)was successfully synthesized.On the 5th day of treatment,the effect of RA-Zn on promoting osteoblast proliferation was superior to that of RA(P＜0.05).RA-Zn of 200 μmol/L had stronger antioxidant capacity than RA(P＜0.05).RA inhibited Staphylococcus aureus activity at the concentration of ≥400 μmol/L(P＜0.05),while RA-Zn showed these inhibitory effect at the concentration of ≥ 25 µmol/L(P＜0.05).Conclusion:The antioxidant and antimicrobial properties are enhanced by the complexation of RA and Zn ions,which promotes osteoclast proliferation.