现代农业研究2024，Vol.30Issue(2)：99-102,4.
小麦叶锈菌2个候选效应因子的克隆及其qRT-PCR分析
Cloning and qRT-PCR Analysis of Two Candidate Effector Proteins Induced by Puccinia Triticina
摘要
Abstract
Wheat leaf rust,caused by Puccinia triticina(Pt)is an airborne fungal disease in wheat worldwide,which can cause significant yield losses to the wheat production in the world.The haustoria of Pt is the main place where the effector proteins were secreted,and the effector protein is the key of the pathogenetic of Pt.The transcriptome librarie of wheat leaf rust races 13-5-28-1(JHKT)was constructed in the stages of dormant spores,spore germination and 6 d infection period.Based on the systematic screening of candidate effectors,two candidate effectors,Pt12448 and Pt34354,were selected for cloning,and the expression patterns of the candidate effectors were analyzed by qRT-PCR.The results showed that the Pt12448 gene was up-regulated in the late induction,and the Pt34354 gene was up-regulated in the early induction.The results of the study will advance the research process of the pathogenic mechanism of P.triticina.关键词
小麦叶锈菌/效应因子/qRT-PCRKey words
wheat leaf rust/effector/qRT-PCR分类
农业科技引用本文复制引用
李建嫄,李林,刘华梁,杜军霞,郭丽..小麦叶锈菌2个候选效应因子的克隆及其qRT-PCR分析[J].现代农业研究,2024,30(2):99-102,4.基金项目
2021年邢台市市级科技计划"邢台市青年人才计划项目"(项目编号:2021ZZ031) （项目编号:2021ZZ031）
