生物技术通报2024，Vol.40Issue(2)：38-47,10.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0787
MDCK细胞无血清悬浮培养技术在流感疫苗研究与生产中的应用进展
The Application Progress of Serum-free Suspension Culture Technology of MDCK Cells in Influenza Vaccine Study and Production
摘要
Abstract
Influenza is a common respiratory disease caused by influenza viruses.MDCK(Madin-Darby Canine Kidney)cells have the characteristics of easy culture and high production,which can support the replication and proliferation of influenza viruses,and are widely used in the research and production of influenza viruses.With the deepening of research on influenza virus,and in order to further optimize the ability of MDCK cells to be used for influenza virus research and industrial application,researchers began to develop the domestication technology of MDCK cells.Through long-term cultivation and selection,the domesticated MDCK cells strain can better adapt to the growth environment of influenza virus,and improve the production and infectivity of influenza virus.In conclusion,the domestication technology of MDCK cells plays an important role in influenza virus research and industrial application,which may improve the production and infectivity of influenza viruses and provide better tools for influenza vaccine production and anti influenza drug development.Concurrently we have to also pay attention to the safety of MDCK cells and take appropriate measures to ensure the safety and reliability of its application.关键词
MDCK细胞/细胞悬浮驯化/无血清培养基/流感疫苗/安全性Key words
MDCK cells/cell suspension demestication/serum-free medium/influenza vaccine/safety引用本文复制引用
靳莉武,张震宇,靳冬武,马花,马玉梅,乔自林,王家敏..MDCK细胞无血清悬浮培养技术在流感疫苗研究与生产中的应用进展[J].生物技术通报,2024,40(2):38-47,10.基金项目
甘肃省青年科技基金计划(22JR11RA239),西北民族大学中央高校基本科研业务费项目(31920230001) （22JR11RA239）
