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水稻热激转录因子HsfA2b调控非生物胁迫抗性的功能分析OA北大核心CSTPCD

Functional Analysis of Rice Heat Shock Transcription Factor HsfA2b Regulating the Resistance to Abiotic Stresses

中文摘要英文摘要

[目的]水稻在生长发育过程中,常会受到各种非生物胁迫而严重影响产量.热激转录因子作为植物抗逆过程中的一个重要元件,通过调控一系列胁迫响应基因的表达以提高植物抗逆性.探究水稻热激转录因子OsHsfA2b调控非生物胁迫的功能与初步机理,为培育水稻抗逆新品种提供了优异基因资源和理论支撑.[方法]通过构建OsHsfA2b过量表达和RNAi的转基因水稻,分别观察水稻幼苗在高温、低温、干旱和高盐处理后的抗逆表型,统计存活率.同时,在逆境胁迫处理后,通过DAB染色检测水稻叶片活性氧(ROS)含量及RT-qPCR检测抗氧化途径相关基因OsSOD和OsCAT的表达量,分析OsHsfA2b对抗氧化途径的调控作用.[结果]在高温、低温、干旱和高盐等非生物胁迫条件下,水稻热激转录因子OsHsfA2b被显著诱导表达.与野生型NPB相比,OsHsfA2b过量表达转基因水稻对非生物胁迫的抗性明显增强,植株损伤程度较轻,存活率提高.相反,OsHsfA2b-RNAi水稻对非生物胁迫更加敏感,植株损伤严重,存活率降低.此外,在非生物胁迫条件下,OsHsfA2b过量表达转基因水稻比NPB和OsHsfA2b-RNAi水稻体内活性氧的含量减少,并且OsHsfA2b显著诱导了抗氧化途径相关基因OsSOD和OsCAT的表达,OsHsfA2b参与抑制水稻体内活性氧的积累以降低逆境胁迫诱导的活性氧大量积累对植株的伤害.[结论]非生物胁迫下,水稻热激转录因子OsHsfA2b被显著诱导表达,可通过抗氧化途径正调控水稻对逆境胁迫的抗性.

[Objective]Rice is often affected by various abiotic stresses during growth and development,which severely restricts rice yield.Heat shock transcription factors(HSFs),as an important element of resistance to abiotic stresses in plant,can enhance plant resistance to abiotic stresses by regulating the expressions of a series of stress related genes.This study aims to investigate the function and mechanism of rice heat shock transcription factor OsHsfA2b regulating abiotic stresses,which may provide excellent gene resource and theoretical support for cultivating new rice varieties with stress resistance.[Method]We constructed OsHsfA2b-overexpressed and RNAi rice transgenic plants,then their seedlings were treated with high temperature,low temperature,drought,and high salt,respectively,and their phenotypes were observed and survival rates were counted.The detection of reactive oxygen species(ROS)deposition by DAB staining,and expression levels of antioxidant pathway related genes OsSOD and OsCAT with RT-qPCR after abiotic stress treatment,aiming to analyze the regulatory effect of OsHsfA2b on the antioxidant pathway.[Result]The rice HSF gene OsHsfA2b was induced significantly by abiotic stress conditions,such as high temperature,low temperature,drought,and high salt.Compared with the wild-type plants,OsHsfA2b-overexpressed transgenic plants significantly enhanced the resistance to abiotic stresses,as well as survival rate,and less damage.On the contrary,both resistance and survival rate of OsHsfA2b-RNAi plants to abiotic stresses decreased,and the plants were severely damaged.Moreover,the ROS deposition in OsHsfA2b-overexpressed plants decreased compared with NPB and RNAi plants under abiotic stress.Correspondingly,OsHsfA2b induced the expressions of antioxidant pathway related genes OsSOD and OsCAT,suggesting that OsHsfA2b suppressed ROS accumulation to reduce its damage caused by abiotic stress induction.[Conclusion]The above results indicate that OsHsfA2b is induced by abiotic stresses and positively regulates rice resistance to abiotic stresses through antioxidant pathways,and it is an excellent gene resource for rice stress-resistance breeding.

邹修为;岳佳妮;李志宇;戴良英;李魏

湖南农业大学植物保护学院, 长沙 410128湖南省临湘市云湖街道农业综合服务中心, 岳阳 414300

水稻HsfA2b热激转录因子非生物胁迫抗性活性氧

Oryza sativa L.HsfA2bheat shock transcription factorabiotic stressresistancereactive oxygen species

《生物技术通报》 2024 (002)

90-98 / 9

湖南省教育厅项目(21A0128),杂交水稻国家重点实验室开放课题(2021KF05)

10.13560/j.cnki.biotech.bull.1985.2023-0908

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