生物技术通报2024，Vol.40Issue(2)：160-171,12.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0856
葡萄CYP707A基因家族的鉴定及对果实成熟的功能验证
Identification and Analysis of Grape(Vitis vinifera L.)CYP707A Gene Family and Functional Verification to Fruit Ripening
摘要
Abstract
[Objective]CYP707A is a subfamily member of cytochrome P450 family,encoding ABA8'-hydroxylase,which is a key enzyme in ABA catabolism and plays an important role in plant growth and development.Identification of grape(Vitis vinifera L.)CYP707A gene family may provide theoretical basis for further study of grape fruit ripening mechanism and biological breeding techniques.[Method]Bioinformatics methods were used to analyze the gene structure,protein motif,chromosome localization,collinearity,phylogenetic relationship and cis-acting elements of promoter region of CYP707A family gene in the grape.RT-qPCR was used to analyze the spatiotemporal expression patterns of VvCYP707As gene in tissues,different grape varieties(early and medium maturity variety)and in response to the exogenous ABA treatment were analyzed by.Fruit transient transfection was applied to verify the function of Vitvi07g01751.[Result]There were five VvCYP707As genes identified in grape genome,located on five chromosomes,among which Vitvi02g01269,Vitvi07g01751 and Vitvi18g00792 were fragment replicators.The CYP707A gene family had a high degree of conservation,sharing similarities in gene structure and protein motifs,and all subcellular localization were predominantly localized to the endoplasmic reticulum.The cis-acting elements of growth,development and hormone response were significantly enriched in the promoter regions of the CYP707A gene family,containing ABA hormone-corresponding elements.Furthermore,the RT-qPCR analysis demonstrated that VvCYP707A genes demonstrated both spatiotemporal-and tissue-specific expression patterns,with elevated expression levels during the early and expansion stages of fruit development as well as in stem and leaf tissues.Notably,Vitvi07g01751 gene was highly expressed in the fruits of the early maturity variety'Yong Zaohong',and the expression pattern was consistent between these two varieties.The expression pattern of Vitvi07g01751first increased and then decreased with fruit development.Under exogenous ABA treatment,VvCYP707A showed two distinct expression patterns:it induced the high expression of 5 VvCYP707A genes in the grape flesh while significantly suppressing the expressions of these genes in the grape pericarp.Furthermore,upon transient expression of Vitvi07g01751 in the grape flesh,there was a slow accumulation of sugar content,accompanied by a significant upregulation of the ABA receptor VvPYL4 gene,suggesting that Vitvi07g01751 mediated ABA regulation of grape fruit ripens.[Cconclusion]Vitvi07g01751 plays an important role in fruit ripening of grape.关键词
葡萄/CYP707A基因/全基因组鉴定/生物信息学/果实成熟/脱落酸/PYL基因Key words
grape/CYP707A gene/genome-wide identification/bioinformatics/fruit ripening/abscisic acid/PYL gene引用本文复制引用
龚丽丽,余花,杨杰,陈天池,赵双滢,吴月燕..葡萄CYP707A基因家族的鉴定及对果实成熟的功能验证[J].生物技术通报,2024,40(2):160-171,12.基金项目
宁波市科技创新 2025 现代种业重大专项(2019B10015),浙江省重点研发计划(2021C02053),生物工程一流学科创新项目(CX2021015) （2019B10015）
