生物技术通报2024，Vol.40Issue(2)：197-211,15.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0830
甘蔗CBL-CIPK基因家族的鉴定和表达分析
Identification and Expression Analysis of the CBL-CIPK Gene Family in Sugarcane
摘要
Abstract
[Objective]Sugarcane is an important sugar crop,and temperature,salinity,water and other factors are the key environmental factors restricting its growth and development.Calcineurin B-Like protein(CBL)family represents a group of Ca2+ binding proteins and plays a key role in decoding calcium signals by specifically interacting with and regulating a family of protein kinases CIPKs(for CBL-interacting protein kinases)in abiotic stress signal transduction pathway.At present,the whole genome of sugarcane has been sequenced,but its CPL-CIPK gene family members have not been identified,and the interaction regulation mechanism is still unknown.Here,the members of CBL and CIPK in sugarcane were identified and the CPL-CIPK interaction relationship was revealed,which may provide gene resources and theoretical basis for studying the mechanism of CPL-CIPK interaction in sugarcane.[Method]The expression levels of CBLs and CIPKs under low temperature,high temperature,NaHCO3 and PEG were analyzed by RT-qPCR from sugarcane GT58.The interactions between SsCBLs and SsCIPKs were analyzed by yeast two-hybrid assay.[Result]There were 19 CBL genes and 82 CIPK genes in the whole genome of sugarcane,which distributed in different evolutionary branches with gene replication.The physicochemical properties of gene family members were different,the structural domains and protein motifs were highly conserved,and the distribution of cis-acting elements was diverse.SsCBL7/SsCBL12/SsCIPK1/SsCIPK5 were specifically expressed under low temperature,high temperature,drought and high salt and other abiotic stress regulation.Meanwhile,SsCBL1 interacted with SsCIPK47 and SsCIPK81,and SsCBL8 interacted with SsCIPK47 and SsCIPK81.[Conclusion]The CBL-CIPK signaling system may play an important role in response to abiotic stress during the growth and development of sugarcane.关键词
甘蔗/SsCBL/SsCIPK/非生物胁迫/酵母双杂交Key words
sugarcane/SsCBL/SsCIPK/abiotic stresses/yeast two-hybrid引用本文复制引用
辛奇,李压凡,尹铮,张晓丹,陈霆,刘晓华..甘蔗CBL-CIPK基因家族的鉴定和表达分析[J].生物技术通报,2024,40(2):197-211,15.基金项目
国家重点研发计划子课题(2019YFD1000503),烟台新旧动能转换研究院暨烟台科技成果转移转化示范基地项目(2020XJDN001) （2019YFD1000503）
