生物技术通报2024，Vol.40Issue(2)：212-222,11.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0896
番茄HMA基因家族的鉴定及SlHMA1镉转运功能研究
Identification of HMA Gene Family and Cadmium Transport Function of SlHMA1 in Tomato
摘要
Abstract
[Objective]The heavy metal ATPase(HMA)gene family is extensively involved in the absorption and transport of metal elements in plants.Therefore,this study is aimed to systematically identify the members of the tomato HMA gene family and their characteristics,as well as to investigate their functions in response to cadmium stress.[Method]Tomato HMA gene family members were identified through bioinformatics,and their systematic evolution tree,protein physicochemical properties,gene structure,cis-regulatory elements,and gene expression patterns,etc.,were analyzed.Additionally,yeast functional complementation experiments were conducted to validate the Cd2+ transport activity of SlHMA1.[Result]Eight SlHMAs were identified in the tomato genome,categorized into 2 subgroups.Remarkable differences in gene structure were observed among SlHMAs genes and their homologs in Arabidopsis and rice.Notably,the promoter regions of SlHMAs members were found to harbor numerous cis-regulatory elements associated with stress responses,and RT-qPCR analysis also indicated that most SlHMAs genes showed tissue-specific responses to cadmium stress.Furthermore,yeast functional complementation experiments provided conclusive evidence for Cd2+ transport activity of SlHMA1,while evolutionary analysis indicated the widespread presence of HMA1 in the plant kingdom,with the motif DKTGT related to ATP hydrolysis activity within HMA1 also being highly conserved across the plant kingdom.[Conclusion]The eight SlHMAs present typical characteristics of the HMA gene family while also having functional diversity.SlHMAs,along with their conserved amino acid motif DKTGT,are closely associated with metal ion transport and cadmium stress response,offering significant potential for application in the breeding of low cadmium-accumulating crop varieties.关键词
番茄/金属转运蛋白/重金属ATP酶/镉/SlHMA1Key words
tomato/metal transporter/heavy metal ATPase/cadmium/SlHMA1引用本文复制引用
赵曜,文朗,骆少丹,栗子杏,刘超超..番茄HMA基因家族的鉴定及SlHMA1镉转运功能研究[J].生物技术通报,2024,40(2):212-222,11.基金项目
国家自然科学基金项目(32002113) （32002113）
