生物技术通报2024，Vol.40Issue(2)：266-276,11.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0837
黄龙病发病芦柑根际土壤细菌群落组成与多样性特征
Bacterial Diversity and Structure in Rhizosphere Soil of Citrus Infested with Huanglongbing
摘要
Abstract
[Objective]This study aims to investigate the rhizosphere soil bacterial community of Citrus reticulata plants in the Nanping region of Fujian province,China.The objective is to analyze the impact of Huanglongbing(HLB)disease on the bacterial community and provide a scientific basis for HLB management strategies in the Nanping region.[Method]We collected root-zone soil samples from both healthy and HLB-affected C.reticulata plants using the five-point sampling method.We used amplicon sequencing technology and bioinformatics methods to investigate the changes in diversity and composition of the bacterial community in the root-zone soil of HLB-affected C.reticulata plants.Additionally,we analyzed the correlation between soil physicochemical factors and the bacterial community.[Result]The findings of the study demonstrate that the diversity of the bacterial community in the root-zone soil of C.reticulata plants affected by HLB was slightly higher when compared to the microbiota of healthy plants.Specifically,there was a significant increase in the relative abundance of the Proteobacteria,and a significant decrease in the relative abundance of the Actinobacteriota in the root-zone soil of HLB-affected C.reticulata plants.At the genus level,it was observed that the relative abundance of the Roseiarcus and Acidiphilium significantly increased in the bacterial community of HLB-affected C.reticulata plants.Furthermore,these genera presented a significant negative correlation with available potassium,available phosphorus,and organic matter,while showing a significantly positive correlation with pH values.Conversely,the relative abundance of the Conexibacter and Chujaibacter were significantly lower in HLB-affected plants than healthy ones.Additionally,these genera demonstrated a significantly positive correlation with available potassium,available phosphorus,and organic matter,but a significant negative correlation with pH values.[Conclusion]The infection of the HLB pathogen altered the physicochemical properties of citrus soil and significantly reduced the abundance of beneficial bacteria in the rhizosphere soil.This,in turn,leads to considerable changes in both the diversity and composition of the rhizosphere bacterial community in C.reticulata plants.Concurrently,HLB-infected C.reticulata plants appear to counteract pathogen invasion by recruiting beneficial bacteria that not only fix nitrogen but also promote plant growth in the rhizosphere.关键词
黄龙病/柑橘/高通量测序/根际土壤/细菌群落/差异物种/环境因子Key words
Huanglongbing/citrus/high-throughput sequencing/rhizosphere soil/bacterial community/differential species/environmental factors引用本文复制引用
雷美玲,饶文华,胡进锋,岳琪,吴祖建,范国成..黄龙病发病芦柑根际土壤细菌群落组成与多样性特征[J].生物技术通报,2024,40(2):266-276,11.基金项目
国家重点研发计划(2021YFD1400800),福建省农业科学院项目(XTCXGC2021011,CXTD2021002-1) （2021YFD1400800）
