生物技术通报2024，Vol.40Issue(2)：277-288,12.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0918
三株无量山森林土壤芽孢杆菌鉴定及其生物活性挖掘
Identification of Three Strains of Bacillus from the Forest Soil of Wuliang Mountain and Mining of Their Bioactivities
摘要
Abstract
[Objective]In order to provide efficient microbial resource for the development of green agricultural inputs,we identified potential high-activity microbial strains from the National Wilderness Preservation System of Mount Wuliangshan.[Method]We used Fusarium oxysporum as a target to screen highly antagonistic strains,and observed the effect on hyphae growth and spore germination.We also tested the lipopeptide synthesis genes and the ability of the strain to produce enzymes,hydrolyze phosphorus,hydrolyze potassium,fix nitrogen and produce siderophore.In addition,the strains were identified according to their morphological,physiological and biochemical characteristics and 16S rRNA,gyrA and rpoB genes,and the effects of disease control and growth promotion were verified by pot experiment.[Result]A total of 153 strains of culturable bacteria were isolated and screened.Among them,the inhibition rates of SH-53,N4471 and N9456 were 92.35%,87.29%and 88.47%,respectively,it showed good antagonistic activity against many pathogens,and also inhibited the hypha growth and conidia germination of the pathogen.The three functional strains all had the abilities of dissolving zinc,producing amylase and protease,and the strains Sh-53 and N4471 also had the abilities of dissolving phosphorus,fixing nitrogen,secreting cellulase and producing siderophore.In addition,we found that srfA,fenA,ituA,ituC,ituD and bymC genes were found in the genomes of the three functional bacteria.Strain SH-53 was identified as Bacillus amyloliquefaciens,N4471 as B.cabrialesii,and N9456 as B.siamensis.The results of pot experiment showed that the control effects of the three functional bacteria were 84.66%,54.96%and 59.74%,respectively.They promoted the agronomic characters of tomato seedlings,such as plant height,stem diameter and root length.[Conclusion]As high-efficiency microbial resources,the three strains have broad-spectrum antibacterial activity,diverse biological activity,and have broad application prospects in the development of green agricultural inputs.关键词
解淀粉芽孢杆菌/卡式芽孢杆菌/暹罗芽孢杆菌/鉴定/生物活性/番茄枯萎病/促生Key words
Bacillus amyloliquefaciens/Bacillus cabrialesii/Bacillus siamensis/identification/biological activity/tomato wilt disease/growth-promoting引用本文复制引用
王楠,杨佩文,廖永琴,施竹凤,申云鑫,杨童雨,冯路遥,矣小鹏,唐加菜,陈齐斌..三株无量山森林土壤芽孢杆菌鉴定及其生物活性挖掘[J].生物技术通报,2024,40(2):277-288,12.基金项目
云南省科技计划重大科技专项(202202AE090010,202202AE090015),国家自然科学基金项目(32060624),云南省专家基层科研工作站-杨佩文专家工作站(嵩明县,陆良县)(云人社通[2022]17 号) （202202AE090010,202202AE090015）
