生物技术通报2024，Vol.40Issue(2)：300-312,13.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0719
雨生红球藻钙依赖蛋白激酶(CDPK)家族鉴定与表达分析
Identification and Expression Analysis of Calcium-dependent Protein Kinase(CDPK)Family in Haematococcus pluvialis
摘要
Abstract
[Objective]Calcium-dependent protein kinases(CDPK or CPK)are widely involved in plant growth and development and environmental stress response.To explore their roles involved in Haematococcus pluvialis growth,development and astaxanthin accumulation,HpCDPK gene family was identified and analyzed.[Method]Bioinformatics tools were used to identify the HpCDPK gene family members,and to analyze the physical and chemical properties,phylogenetic evolution,conserved motifs and functional domains of the encoded proteins,as well as the gene expression profiles under nitrogen deficiency stress.[Result]The results showed that seven members of HpCDPK gene family were identified,and all HpCDPK proteins contained typical EF-hand motifs and protein kinase domains.Phylogenetic analysis showed that these HpCDPKs clustered with the CrCDPKs from Chlamydomonas reinhartii and separated from the AtCDPKs of Arabidopsis thaliana,suggesting that species-specific gene replication occurred in the process of evolution.Transcriptome data analysis showed that HpCDPK gene expression was induced by a variety of stresses,among which the transcription level of HpCDPK7 gene was most significantly up-regulated under nitrogen deficiency.In addition,the analysis of the correlation between HpCDPK and the expression of genes related to carotenoid synthesis showed that HpCDPK was closely related to the expressions of genes relevant to several carotenoid synthesis in H.pluvialis,especially HpCDPK2 was positively correlated with the expression of key genes of astaxanthin synthesis(BKT and BCH).[Conclusion]Seven HpCDPK genes were identified,the expression of HpCDPK7 gene was most significantly up-regulated under nitrogen deficiency and HpCDPK2 may play an active role in the synthesis and accumulation of carotenoids and astaxanthin.The results provide a reference basis for further analysis of the function and molecular mechanism of stress response and carotenoids synthesis and accumulation mediated by HpCDPK in H.pluvialis.关键词
雨生红球藻/钙依赖性蛋白激酶/序列分析/表达模式/非生物逆境Key words
Haematococcus pluvialis/calcium-dependent protein kinase/sequence analysis/expression pattern/abiotic stress引用本文复制引用
李亚男,崔红利,张豪杰,梁梦静,罗涛,李旺宁,张春辉,季春丽,李润植,薛金爱..雨生红球藻钙依赖蛋白激酶(CDPK)家族鉴定与表达分析[J].生物技术通报,2024,40(2):300-312,13.基金项目
国家重点研发计划项目(2021YFD1901105),国家自然科学基金项目(31902394 和 41876188),山西省高等学校科技创新计划(2021L119),山西农业大学科技创新基金(2018YJ16) （2021YFD1901105）
