中国组织工程研究2024，Vol.28Issue(31)：4921-4925,5.DOI:10.12307/2024.708
Pax6基因表达对过氧化氢诱导骨髓间充质干细胞衰老的影响
Effect of Pax6 gene expression on hydrogen peroxide-induced aging in bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND:The occurrence and development of various ophthalmic diseases are closely related to excessive oxidative stress,and the inhibition of oxidative stress response may produce preventive and therapeutic effects. OBJECTIVE:To explore the role of Pax6 gene expression on hydrogen peroxide-induced aging of mouse bone marrow mesenchymal stem cells(BM-MSCs). METHODS:Resuscitated BM-MSCs,Pax6/BM-MSCs,and shPax6/BM-MSCs were treated with hydrogen peroxide for 24 hours,and then β-galactosidase staining was performed.The proliferation index Ki67 expression and apoptosis were detected by flow cytometry.The expression of senescence-associated molecules(Wnt7a,p21,and p53)was detected by RT-PCR. RESULTS AND CONCLUSION:(1)After hydrogen peroxide treatment,the cells of the three groups showed senescence phenotype and β-galactosidase staining was positive.Compared with BM-MSCs group,the expression of positive cells in Pax6/BM-MSCs group was less and that in the shPax6/BM-MSCs group was more,and the difference was statistically significant(P<0.05).(2)The results of flow cytometry showed that compared with BM-MSCs group,the positive expression of Ki67 in the Pax6/BM-MSCs group increased and the level of apoptosis decreased,while the positive expression of Ki67 decreased and the level of apoptosis increased in the shPax6/BM-MSCs group;the difference was significantly different(P<0.05).(3)RT-PCR showed that compared with the BM-MSCs group,the expression of Wnt7a,p53,and p21 decreased in the Pax6/BM-MSCs group,while the expression of Wnt7a,p53,and p21 increased in the shPax6/BM-MSCs group;the difference was significantly different(P<0.05).(4)These findings indicate that overexpression of Pax6 can antagonize the aging progression of BM-MSCs induced by hydrogen peroxide,which may be related to Wnt signaling pathway.关键词
骨髓间充质干细胞/Pax6/Wnt7a/过氧化氢/凋亡/衰老Key words
bone marrow mesenchymal stem cell/Pax6/Wnt7a/hydrogen peroxide/apoptosis/aging分类
医药卫生引用本文复制引用
高杰,邹星星,文邦红,李远迪,苏敏,胡蓉..Pax6基因表达对过氧化氢诱导骨髓间充质干细胞衰老的影响[J].中国组织工程研究,2024,28(31):4921-4925,5.基金项目
国家自然科学基金(82171343,82060151),项目负责人:苏敏 （82171343,82060151）
贵州省科技厅项目(黔科合支撑[2020]4Y230号),项目负责人:苏敏 （黔科合支撑[2020]4Y230号）
贵州省科技厅项目(黔科合基础-ZK[2022]一般344),项目负责人:高杰 （黔科合基础-ZK[2022]一般344）
贵州省高等学校重点实验室(黔教技[2023]016号),项目负责人:苏敏National Natural Science Foundation of China,No.82171343,82060151(to SM) （黔教技[2023]016号）
Project of Guizhou Science and Technology Department,No.[2020]4Y230(to SM) （to SM）
Project of Guizhou Science and Technology Department,No.[2022]344(to GJ) （to GJ）
Key Laboratory of Higher Learning Schools of Guizhou Province,No.[2023]016(to SM) （to SM）
