中国组织工程研究2024，Vol.28Issue(31)：4945-4950,6.DOI:10.12307/2024.707
槲皮素靶向CCR1、CXCR4促进人骨髓间充质干细胞的迁移
Quercetin targets CCR1 and CXCR4 to promote migration of human bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND:Quercetin plays an important role in the proliferation and differentiation of bone marrow mesenchymal stem cells,but less research has been done on its mechanism of promoting the migration of bone marrow mesenchymal stem cells. OBJECTIVE:To study the effect of quercetin on the migration of human bone marrow mesenchymal stem cells through in vitro experiments,and to explore the regulatory role of CCR1 and CXCR4. METHODS:Human bone marrow mesenchymal stem cells were selected as experimental subjects.CCK8 assay was used to detect the effect of quercetin on the proliferative activity of human bone marrow mesenchymal stem cells.Cell scratch assay and Transwell assay were used to detect the in vitro invasive and migratory abilities of human bone marrow mesenchymal stem cells after quercetin treatment,respectively.The role of quercetin in relation to CCR1 and CXCR4 was demonstrated with the help of molecular docking technology.Western blot assay and real-time fluorescence quantitative PCR were used to detect the migration-related chemokine expression after quercetin treatment. RESULTS AND CONCLUSION:(1)5 and 10 μmol/L quercetin could significantly promote the proliferation of human bone marrow mesenchymal stem cells,and the drug concentration of 10 μmol/L resulted in the highest cell proliferation efficiency.(2)To better explore the dose-effect relationship of quercetin affecting the migration of human bone marrow mesenchymal stem cells,5 and 10 μmol/L quercetin were selected for the subsequent experiments,and ligustrazine was used as the positive control drug,and the experiments were divided into blank control group,5 μmol/L quercetin group,10 μmol/L quercetin group,and 100 μmol/L ligustrazine group.(3)In vitro migration and invasion ability of human bone marrow mesenchymal stem cells were elevated in a concentration-dependent manner after quercetin treatment,and the migration effect of 10 μmol/L quercetin group was better than that of ligustrazine group.(4)The molecular docking results suggested that there was a strong interaction between quercetin and CCR1 and CXCR4.(5)Quercetin could up-regulate the expression of CCR1 and CXCR4 proteins and mRNA.(6)This study confirmed at the cellular level that quercetin could promote the migration of human bone marrow mesenchymal stem cells by targeting CCR1 and CXCR4.关键词
骨髓间充质干细胞/槲皮素/迁移/增殖/分子对接Key words
bone marrow mesenchymal stem cell/quercetin/migration/proliferation/molecular docking分类
医药卫生引用本文复制引用
陈双,席志鹏,王楠,房晓阳,刘鑫,康然,谢林..槲皮素靶向CCR1、CXCR4促进人骨髓间充质干细胞的迁移[J].中国组织工程研究,2024,28(31):4945-4950,6.基金项目
江苏省干部保健科研课题(BJ19030),项目负责人:席志鹏 （BJ19030）
江苏省基础研究计划自然科学基金项目(BK20221420),项目负责人:谢林Jiangsu Province Cadre Health Research Project,No.BJ19030(to XZP) （BK20221420）
Natural Science Foundation of Jiangsu Basic Research Program,No.BK20221420(to XL) （to XL）
