中国组织工程研究2024，Vol.28Issue(31)：4964-4969,6.DOI:10.12307/2024.710
过表达长链非编码RNA Gm16104影响C3H10T1/2间充质干细胞的成骨分化
Overexpression of long non-coding RNA Gm16104 affects osteogenic differentiation of C3H10T1/2 mesenchymal stem cells
摘要
Abstract
BACKGROUND:The osteogenic differentiation of mesenchymal stem cells is regulated by a variety of molecules.Long non-coding RNA(lncRNA)has attracted much attention because they can participate in regulating a variety of biological processes,but the regulatory role of lncRNA on osteogenic differentiation of mesenchymal stem cells has not been fully explored. OBJECTIVE:To explore the effect of lncRNA Gm16104 on osteogenic differentiation of C3H10T1/2 mesenchymal stem cells. METHODS:The C3H10T1/2 mesenchymal stem cells were induced into osteogenic differentiation by bone morphogenetic protein-2.Alkaline phosphatase staining was performed to identify the osteogenic differentiation of the cells 5 days after osteogenic induction.Expression levels of alkaline phosphatase and lncRNA Gm16104 were detected by qRT-PCR after 0,1,3,and 5 days of osteogenic differentiation.After transfection of the overexpression plasmid of pcDNA-Gm16104,the osteogenic differentiation was identified by alkaline phosphatase staining and qRT-PCR 4 days after osteogenic induction.The expression levels of osteogenesis-related signalling pathway proteins were detected by western blot assay. RESULTS AND CONCLUSION:(1)After 5 days of osteogenic induction,alkaline phosphatase activity was significantly increased.(2)Compared with 0 days,expression levels of the osteogenic marker gene alkaline phosphatase increased and expression levels of Gm16104 decreased after 1,3,and 5 days of osteogenic induction.(3)Transfection of C3H10T1/2 cells with pcDNA-Gm16104 plasmid significantly increased the expression level of Gm16104.(4)Overexpression of Gm16104 inhibited alkaline phosphatase activity,the expression levels of the osteogenic marker gene alkaline phosphatase and the osteogenesis-related transcription factor Osterix.(5)Overexpression of Gm16104 inhibited phosphorylated protein expression of PI3K and Akt.(6)The above results suggest that overexpression of Gm16104 may inhibit osteogenic differentiation of C3H10T1/2 mesenchymal stem cells through the PI3K/Akt signaling pathway.关键词
长链非编码RNA/间充质干细胞/Gm16104/骨形态发生蛋白2/成骨分化/PI3K/AktKey words
long non-coding RNA/mesenchymal stem cell/Gm16104/bone morphogenetic protein-2/osteogenic differentiation/PI3K/Akt分类
医药卫生引用本文复制引用
林展莹,林紫云,黄柳艳,张文烯,左长清..过表达长链非编码RNA Gm16104影响C3H10T1/2间充质干细胞的成骨分化[J].中国组织工程研究,2024,28(31):4964-4969,6.基金项目
省级大学生创新创业训练项目(S202210571052),项目负责人:林紫云 （S202210571052）
广东省自然科学基金面上项目(2017A030313641,2018A0303130203),项目负责人:左长清Provincial Innovation Training Program for College Students,No.S202210571052(to LZY) （2017A030313641,2018A0303130203）
Guangdong Provincial Natural Science Foundation(General Program),No.2017A030313641,No.2018A0303130203(to ZCQ) （General Program）
