畜牧与兽医2024，Vol.56Issue(3)：1-5,5.
线粒体tRNA-Lys(T7719G)基因变异对绵羊卵巢颗粒细胞生理功能的影响
Effects of mitochondrial tRNA-Lys(T7719G)gene variations on the physiological function of granulosa cells in sheep
摘要
Abstract
Granulosa cells are important components of the follicle,and they secrete a variety of factors required for follicular development to regulate oocyte growth,differentiation,maturation and atresia.Studies have shown that mitochondrial tRNA variants alter physiological functions such as mitochondrial fusion division and autophagy.In this study,genotypes were determined by sequencing PCR amplification products from ovarian tissues of sheep,and apoptosis was measured by flow cytometry.The results showed that there were two genotypes of mitochondrial tRNA-Lys gene,G and T.The apoptosis rate of the granulosa cells of genotype G was significantly lower(P＜0.05).The con-tent of division-related protein MFF was significantly higher(P＜0.05),and mitochondrial fission was enhanced in genotype G samples.Taken together,mitochondrial tRNA-Lys(T7719G)significantly reduced the rate of apoptosis,increased the value of the division-related protein MFF,and promoted and increased cell division.关键词
线粒体基因组/绵羊/颗粒细胞/生理功能Key words
mitochondrial DNA/sheep/granular cells/physiological function分类
农业科技引用本文复制引用
吕世琪,马晓菲,周荣艳,陈晓勇..线粒体tRNA-Lys(T7719G)基因变异对绵羊卵巢颗粒细胞生理功能的影响[J].畜牧与兽医,2024,56(3):1-5,5.基金项目
河北省自然科学基金项目(C2020204001) （C2020204001）
