中国组织工程研究2024，Vol.28Issue(31)：5042-5047,6.DOI:10.12307/2024.701
间充质干细胞来源外泌体及衍生miRNA治疗病理性瘢痕的作用机制
miRNA derived from mesenchymal stem cells and its derivatives in treatment of pathological scar
摘要
Abstract
BACKGROUND:Stem cell therapy has become an emerging method of treating pathological scars.miRNAs are involved in scarring mechanisms,and the targeted action of some stem cell sources of miRNA is mediated by exosomes. OBJECTIVE:To review the biological properties of miRNA derived from mesenchymal stem cells and its derivatives,the mechanism of treatment of scarring through anti-inflammation,suppression of excessive tissue reconstruction and antioxidation. METHODS:The first author used a computer in September 2023 to retrieve the relevant literature published from January 2000 to September 2023,searching for"stem cell,exosome,miRNA,keloid"in English,and"stem cells,exosome,keloid"in Chinese,eventually incorporating 74 papers for analysis. RESULTS AND CONCLUSION:(1)miRNAs with high expression of exosomes derived from mesenchymal stem cells increase the proportion of M2-type macrophages by promoting the polarization of macrophages,target the regulation of transforming growth factor β,transforming growth factor β receptors or related signaling pathways,inhibit the expression of pro-inflammatory factors,promote the expression of anti-inflammatory factors and other mechanisms to inhibit inflammation and thus suppress scar lesions.(2)miRNAs with high expression of exosomes derived from mesenchymal stem cells can reduce the secretion of matrix metalloproteinases,regulate the balance between matrix metalloproteinase inhibitors and matrix metalloproteinases,inhibit the proliferation and migration of fibroblasts and myofibroblasts,directly reduce the production of collagen and other mechanisms,and ultimately lead to the normal degradation of extracellular matrix,thereby inhibiting excessive tissue remodeling and cicatricial lesions.(3)miRNAs with high expression of exosomes from mesenchymal stem cells can improve the resistance of scar fibroblasts to oxidative stress by regulating reactive oxygen species and hypoxia-inducing factors,and then regulate the proliferation and apoptosis of scar fibroblasts to inhibit scar lesions.(4)Exosomes derived from mesenchymal stem cells have good prospects for scar treatment.Studies on this aspect can find mirnas that regulate inflammatory cells,inflammatory factors,signaling pathways,matrix metalloproteinases,fibroblasts,reactive oxygen species,hypoxia-inducing factors and other key factors from the three aspects of inflammation,tissue remodeling and oxidative stress.Then,by inducing mesenchymal stem cells with high expression of the above miRNA,exosomes were extracted,and finally verified and clinical trials were carried out.关键词
间充质干细胞/外泌体/miRNA/病理性瘢痕/炎症/组织重塑/氧化应激Key words
mesenchymal stem cell/exosome/miRNA/pathological scar/inflammation/tissue remodeling/oxidative stress分类
医药卫生引用本文复制引用
李玥,乔华..间充质干细胞来源外泌体及衍生miRNA治疗病理性瘢痕的作用机制[J].中国组织工程研究,2024,28(31):5042-5047,6.基金项目
山西省高等学校大学生创新创业训练计划项目(20230295),项目负责人:李玥 （20230295）
山西省科技厅青年科技研究基金(201901D211339),项目负责人:乔华Innovation and Entrepreneurship Training Program for College Students in Shanxi Province,No.20230295(to LY) （201901D211339）
Youth Science and Technology Research Fund of Shanxi Provincial Department of Science and Technology,No.201901D211339(to QH) （to QH）
