畜牧与兽医2024，Vol.56Issue(3)：39-44,6.
虾青素缓解玉米赤霉烯酮诱导的IPEC-J2细胞损伤效果研究
Effects of astaxanthin on zearalenone induced injury of IPEC-J2 cells
摘要
Abstract
The aim of this experiment was to investigate the effect of astaxanthin on zearalenone(ZEN)-induced damage in porcine small intestinal epithelial cells(IPEC-J2 cells).The experimental groups were:Group C(treated with normal medium),Group Z(with 25 μg/mL ZEN),Group ZA1(with 25 μg/mL ZEN+5 μmol/L astaxanthin),Group ZA2(with 25 μg/mL ZEN+10 μmol/L astaxanthin)and Group ZA3(with 25 μg/mL ZEN+20 μmol/L astaxanthin).The cell viability,cellular lactate dehydrogenase(LDH)activity,cyto-kine(IL-4,IL-6 and TNF-α)level,antioxidant enzyme levels and mRNA expressions of the endoplasmic reticulum stress-related genes were measured.The results showed that the cell viability,SOD activity,GSH-Px activity,T-AOC level,IL-4 level,Bcl-2 and Bcl-XL gene mRNA expression levels in Group Z were lower than those in Group C(P＜0.01);the cell viability,GSH-Px activity,T-AOC level,IL-4 level,Bcl-2 and Bcl-XL gene mRNA expression levels in Groups ZA1,ZA2 and ZA3 were higher than those in Group Z(P＜0.01 or P＜0.05).The LDH activity,MDA level,IL-1 level,IL-6 level,TNF-α level and ATF6,CHOP and BAX gene mRNA expression levels,and the value of BAX/Bcl-2 in Group Z were higher than those in Group C(P＜0.01).The LDH activity,MDA level,IL-1 level,IL-6 level,TNF-α level and ATF6,CHOP and BAX gene mRNA expression levels,and the value of BAX/Bcl-2 in Groups ZA1,ZA2 and ZA3 were lower than those in Group Z(P＜0.01 or P＜0.05).Taken together,astaxanthin counteracted the ZEN induced changes of cell viability,LDH activity,antioxidant function,and the mRNA expression of endoplasmic reticulum stress-related genes of IPEC-J2 cells.关键词
虾青素/ZEN/IPEC-J2细胞/乳酸脱氢酶活性/细胞因子/抗氧化功能/内质网应激Key words
astaxanthin/ZEN/IPEC-J2 cells/lactate dehydrogenase activity/cytokines/antioxidant function/endoplasmic reticulum stress分类
农业科技引用本文复制引用
刘钦宇,佘福泽,顾依萍,杨年溥,秦顺义..虾青素缓解玉米赤霉烯酮诱导的IPEC-J2细胞损伤效果研究[J].畜牧与兽医,2024,56(3):39-44,6.基金项目
国家级大学生创新创业计划训练项目(202210061006) （202210061006）
天津市生猪产业技术体系创新团队项目(ITTPRS2022006) （ITTPRS2022006）
天津市自然科学基金重点项目(20ZYCGSN00260) （20ZYCGSN00260）
