西北植物学报2024，Vol.44Issue(1)：1-12,12.DOI:10.7606/j.issn.1000-4025.20230500
荷花PIN基因家族的鉴定及非生物胁迫表达分析
Genome-wide identification and expression pattern of the PIN gene family under abiotic stress in Nelumbo nucifera
摘要
Abstract
[Objective]To characterize NnPIN gene family in lotus(Nelumbo nucifera)and analyze their roles in abiotic stress,in order to provide genetic resources for the breeding of new lotus varieties with stress resistance.[Methods]This study used bioinformatics methods to conduct a genome-wide identifica-tion of the NnPIN gene family in lotus,and used qRT-PCR to analyze their response to low temperature,flooding,and exogenous application of abscisic acid(ABA).[Results](1)A total of 12 NnPIN genes with transmembrane domains were identified in lotus genome,named NnPIN1-12 respectively,which were distributed on 6 chromosomes,and were mainly located in the plasma membrane and endoplasmic re-ticulum.(2)Phylogenetic analysis showed that NnPIN proteins can be divided into two types:Classical and non-classical based on the length of the central hydrophilic ring,and the same type had a similar struc-ture.The classic type had complete motifs 1 to 12,while the non-classic type missed the central hydro-philic ring(motif 6,8,9,12).(3)Cis-acting element analysis found that the NnPIN genes have a large number of light,abiotic stress and hormone responsive elements in the promoter region,specifically hav-ing anaerobic,ABA,low temperature,and other response elements.(4)The NnPIN genes family,ex-cept NnPIN8,responded positively to exogenous ABA and low temperature stress.In addition,NnPIN6 and NnPIN7 positively responded to flooding stress,while NnPIN1-5,NnPIN8,NnPIN9,and NnPIN12 responded negatively to flooding stress.[Conclusion]The NnPIN gene family members are specifically involved in response to abiotic stress in lotus.关键词
荷花/PIN基因家族/生物信息学/非生物胁迫/表达模式Key words
lotus/PIN gene family/bioinformatics/abiotic stress/expression pattern分类
农业科技引用本文复制引用
程志鹏,汪仲毅,匡健华,赵晗茜,陈龙清,胡慧贞..荷花PIN基因家族的鉴定及非生物胁迫表达分析[J].西北植物学报,2024,44(1):1-12,12.基金项目
国家自然科学基金地区科学基金项目(32360070) （32360070）
云南省科技厅重大科技专项计划(202202AE090028) （202202AE090028）
云南省高层次人才引进计划高端外国专家专项(YNQR-GDWG-2020-001) （YNQR-GDWG-2020-001）
云南省农业基础联合面上项目(202101BD070001-124) （202101BD070001-124）
云南省教育厅科学研究基金项目(2023Y0748) （2023Y0748）
