畜牧与兽医2024，Vol.56Issue(3)：45-53,9.
猪源和犬源ELF4蛋白的亚细胞定位比较研究
Comparative study on subcellular localization of swine-and canine-derived ELF4 protein
摘要
Abstract
This study was to compare and analyze the subcellular localization of swine E74-like factor 4(sELF4)and canine E74-like fac-tor 4(cELF4),so as to provide a basis for further functional research on the two proteins.PCR was used here to truncate sELF4 and cELF4,which were cloned into the eukaryotic expression vector pEGFP-C1,separately.17 sELF4 recombinant eukaryotic expression vectors and 13 cELF4 recombinant eukaryotic expression plasmids were constructed,transfected into HeLa cells and stained with Hoechst33342.Subcellular localization was observed by fluorescence inverted microscope,and bioinformatics software was used to analyze the sequence characteristics of encoded amino acids.The results showed that the full length of porcine and canine ELF4 proteins was localized in the nucleus.There might be two cytoplasmic nuclear localization signal peptides between sELF4 196-291aa(586-873 bp),and the nuclear localization signal peptide of cELF4 was distributed between 203-291 aa(607-873 bp).CELF4 292-663aa(874-1 992 bp)and sELF4 292-662aa(874-1 989 bp)were aggregated in the cells.The amino acid sequences of cELF4 and sELF4 were highly conserved between amino acids 169-303aa,and they were of different degrees in other parts.Taken together,canine ELF4 protein and porcine ELF4 protein were both localized in the nucle-us,and they seemed to aggregate in the truncated expression of the carboxyl terminal functional region.The distribution of nuclear localization signal peptides between them varied.关键词
猪ELF4蛋白/犬ELF4蛋白/真核表达/亚细胞定位Key words
swine ELF4 protein/canine ELF4 protein/eukaryotic expression/subcellular localization分类
农业科技引用本文复制引用
高翠翠,朱钰英,危艳武,喻娇,唐青海,侯鑫军,刘婷,全飞杨,刘建行,赵婷芳,赵铖..猪源和犬源ELF4蛋白的亚细胞定位比较研究[J].畜牧与兽医,2024,56(3):45-53,9.基金项目
国家级大学创新创业训练项目(cxcy2022001) （cxcy2022001）
湖南省自然科学基金面上项目(2021JJ30060) （2021JJ30060）
湖南省教育厅科学研究项目重点项目(21A0442) （21A0442）
湖南省重点研发计划项目(2021NK2026) （2021NK2026）
2022年中央引导地方科技发展资金项目(2022ZYC091) （2022ZYC091）
