畜牧与兽医2024，Vol.56Issue(3)：62-68,7.
依福地平对猪流行性腹泻病毒的抑制作用研究
Inhibition of efonidipine on porcine epidemic diarrhea virus
摘要
Abstract
Porcine epidemic diarrhea virus(PEDV)is the causative agent of porcine epidemic diarrhea(PED).In this study,CCK-8,Cell-based ELISA,Western blot,Time-of-addition assay and viral binding and entry assay were performed to determine the inhibitory effect and molecular mechanism of efonidipine on PEDV infection.The results showed that efonidipine had low cytotoxicity on Vero-E6 and Marc-145 cells within the concentration of 20 μmol/L.Meanwhile,the Western blot results showed that efonidipine significantly inhibited the ex-pression level of the PEDV-N protein on the susceptible cells,mainly inhibiting the entry stage of PEDV.Also,efonidipine was able to in-hibit the infection of host cells with herpes simplex virus type Ⅰ(HSV-1)and porcine pseudorabies virus(PRV),making it a potential broad-spectrum antiviral drug.关键词
猪流行性腹泻病毒/依福地平/病毒入胞/广谱抗病毒效应Key words
PEDV/efonidipine/viral entry/broad-spectrum antiviral effect分类
农业科技引用本文复制引用
张财盛,陈欢,司安琪,赵普,齐传翔,钱莺娟,郑龙三,戴建君..依福地平对猪流行性腹泻病毒的抑制作用研究[J].畜牧与兽医,2024,56(3):62-68,7.基金项目
国家自然科学基金项目(U19A2039,31900141) （U19A2039,31900141）
中央高校基本业务费项目(KJQN202022) （KJQN202022）
江苏省高校优势学科建设工程项目(PAPD) （PAPD）
