Abstract
In order to understand the prevalence and molecular characteristics of porcine rotavirus(PoRV)in some pig farms in China,in the period from 2021 to 2022,6 472 diarrhea samples were collected from 86 large-scale pig farms in some provinces of China and were studied.The prevalence of PoRV was investigated using RT-PCR,and the VP7 and VP4 genes in the PoRV positive samples were amplified and sequenced.Then,homology analysis was performed using the MegAlign software,and a phylogenetic tree was constructed using MEGA11.0.A total of 1 805 out of 6 472 diarrhea samples were positive for PoRV,with a positive rate of 27.89%(1 805/6 472)of all the samples and 65.12%(56/86)in the pig farms.76 VP7 fragments were amplified from 98 positive PoRV samples,G9(42.11%)was the dominant genotype,followed by G5,G26,G3,G4,G1,G2,and G11 which accounted for 26.32%,9.21%,6.58%,6.58%,3.95%,2.63%,and2.63%of the samples,respectively.The P[13]genotype(57.14%)was dominant among the 35 P types,followed by P[7],P[23],P[3],and P[6]types,accounting for 20%,14.29%,5.71%,and 2.86%,respectively.Furthermore,the G/P com-bination genotype was successfully identified in the 35 strains,and the dominant combination genotype was G9P[13](28.57%),the other weaker combinations were G5P[13](11.43%),G4P[13](8.57%),G9P[23](8.57%),G26P[13](5.71%),G1P[7](5.71%),G26P[3](5.71%),G5P[7](5.71%),G3P[7](2.86%),G11P[23](2.86%),G5P[23](2.86%),G11P[7](2.86%),G3P[13](2.86%),G5P[6](2.86%),and G4P[7](2.86%).Obviously,G11P[7]was first identified in China.Taken together,the current prevalence of PoRV was high and the genotypes were complex,with the dominant combina-tion genotype being G9P[13].The results of this study enriched the molecular epidemiology data of PoRV,which would be of great signifi-cance for the prevention and control of PoRV infection in pigs in China,and would also provide reference for the development of new vac-cines.关键词
猪轮状病毒/VP4基因/VP7基因/分子流行病学Key words
porcine rotavirus/VP4 gene/VP7 gene/molecular epidemiology分类
农业科技
