畜牧与兽医2024，Vol.56Issue(3)：86-93,8.
非洲猪瘟病毒CD2v蛋白单克隆抗体的制备与鉴定
Development and characterization of monoclonal antibodies against African swine fever virus protein CD2v
摘要
Abstract
In this study,eight hybridoma cell lines which were capable of stably secreting monoclonal antibodies against CD2v protein,named RH1,RH3,RH4,RH5,RH6,RH8,RH9 and RH10,were obtained by inoculating BALB/c mice with the prokaryotic recombinant CD2v intramembrane region protein of African swine fever virus,and followed by cell fusion,indirect ELISA screening and multiple subclon-ing.The light chain type of the eight monoclonal antibodies was Kappa type.Three of the monoclonal antibodies,RH1,RH5 and RH9,were of IgG2b heavy chain subtype and the remaining five monoclonal antibodies were of IgG1 heavy chain subtype.The eight monoclonal antibod-ies showed good reactivity and specifically recognized the recombinant CD2v protein expressed by the insect baculovirus system,which might be important biological material for the functional elucidation of ASFV structural proteins and the development of serological diagnostic meth-ods.关键词
非洲猪瘟病毒/CD2v蛋白/单克隆抗体Key words
ASFV/CD2v protein/monoclonal antibody分类
农业科技引用本文复制引用
陈艳,祝贺,童明龙,陆冠亚,茹小桐,姜焱,周斌,龙云凤..非洲猪瘟病毒CD2v蛋白单克隆抗体的制备与鉴定[J].畜牧与兽医,2024,56(3):86-93,8.基金项目
南京海关科技计划项目(2021KJ05) （2021KJ05）
