畜牧与兽医2024，Vol.56Issue(3)：99-105,7.
基于16S rDNA测序分析柔嫩艾美耳球虫感染对鸡盲肠菌群的影响
Analysis of cecal microbiota in chickens infected with Eimeria tenella based on the 16S rDNA sequencing technology
摘要
Abstract
To explore the effect of Eimeria tenella infection on the cecal flora of chickens,the cecal contents in chickens in the control group and the infected group were collected and their DNA was extracted.16S rDNA sequencing was used to analyze the changes in the abun-dance and diversity of the cecal flora.The 16S rDNA sequencing results showed that,compared with the control group,the abundance and diversity of bacteria in the cecum of the treated group were significantly reduced.At the phylum level,the dominant microbiota in the control group and the treat group were Firmicutes,Proteobacteria,and Actinobacteriota.After Eimeria tenella infection,the abundance of Firmicutes decreased,and those of Proteobacteria and Actinobacteriota were increased,the difference was significant(P＜0.05).At the same time,the dominant Bacteroidot appeared in the treated group.At the genus level,the abundances of Lactobacillus,Escherichia-Shigella,Bacteroides,Rombutes,Corynebacterium,Enterococcus and Proteus in the cecal microflora of the chickens in the treated group was increased,compared with that of the control group,but the difference was not significant(P＞0.05).The abundances of f-Lachnospiraceae_unclassified,the[Ru-minococcus]_torques_group,Clostridia_UCG-014,f-Ruminococcaceae_unclassified were decreased significantly(P＜0.05).GCA-900066575 was also decreased but not significantly(P＞0.05).The results of the fecal microbiota transplantation showed that the cecal content transplantation group and the fecal bacteria transplantation group significantly reduced the weight gain of the chickens infected with Eimeria tenella,alleviated the cecal injury and OPG significantly,as compared with the saline transplantation group.The anticoccidial inde-xes of the cecal contents transplantation group and the fecal microbiota transplantation group were 173.12 and 164.38,respectively.The a-bove results indicated that Eimeria tenella infection destroyed the original intestinal flora,changed the composition and structure of the chick-en cecum,and promoted the rapid growth and colonization of pathogenic bacteria represented by Escherichia-Shigella,while the presence of probiotics in intestines of normal chickens intestines protect them against Eimeria tenella infection.关键词
鸡/柔嫩艾美耳球虫/肠道菌群/16S rDNA测序/粪菌移植Key words
chicken/Eimeria tenella/intestinal flora/16S rDNA sequencing/fecal microbiota transplantation分类
农业科技引用本文复制引用
刘剑华,张光际,袁橙,卢妍彤..基于16S rDNA测序分析柔嫩艾美耳球虫感染对鸡盲肠菌群的影响[J].畜牧与兽医,2024,56(3):99-105,7.基金项目
江苏省泰州市"凤城英才"青年科技人才托举工程(1710021005) （1710021005）
江苏高校"青蓝工程"资助项目(苏教师函[2020]10号) （苏教师函[2020]10号）
江苏农牧科技职业学院项目(NSF2022ZR08) （NSF2022ZR08）
