诊断学理论与实践2023，Vol.22Issue(5)：466-471,6.DOI:10.16150/j.1671-2870.2023.05.008
同种特异性自身抗体的血清学特点分析及输血策略的探讨
Analysis of the serological characteristics and transfusion strategy of the autoantibody with specificity
摘要
Abstract
Objective:To explore the detection methods and transfusion strategies of autoantibodies with specific-ity against Rh blood group system to provide timely and effective transfusion treatment for those patients.Methods:A case of a patient with autoantibodies with anti-Ce specificity was studied through serological tests,including ABO and Rh blood group identification,Coombs tests,antibody identification test and acid elution test.PCR-SSP was carried out to identify the Rh phenotype.The cross-matching test should avoid the specificity of anti-Ce autoantibody.The hemoglobin was measured pre-and post-the transfusion.Results:The results of the serological tests showed the blood group of the patient was AB type,RhD positive,and Rh blood group was CcEe before transfusion Direct antiglobulin test(DAT),and indirect antiglobu-lin test(IAT)were positive.According to the identification of antibodies,there were autoantibodies with anti-Ce specificity in the patient's serum.The Rh antigen phenotyping was C+,c+,E+,e+ through the gene analysis.The results of the cross-matching were negative and the blood group of the red blood cell were AB,ccDEE.The hemoglobin of the patient increased from 48 g/L to 60 g/L and 58 g/L to 88 g/L rapidly after transfusion.Conclusions:In rare cases,autoantibodies can also show the characteristics of the complete antibodies rather than the pattern which could be agglutination with all the red blood cells for antibodies identification but there were strength differences.During blood transfusion treatment for those patients,we should try to avoid the specificity of autoantibodies,and carry out cross matching test before transfusion.After the titter of the autoantibodies decreased or disappeared,homotypic red blood cells for transfusion could be carried out.关键词
自身抗体/同种抗体/抗Ce抗体/输血治疗Key words
Autoantibody/Alloantibody/Anti-Ce antibody/Treatment with transfusion分类
医药卫生引用本文复制引用
雷航,王中英,谢军华,龚国琴,向东,蔡晓红,邹纬..同种特异性自身抗体的血清学特点分析及输血策略的探讨[J].诊断学理论与实践,2023,22(5):466-471,6.基金项目
中国输血协会威高科研基金(CSBT-WG-2019-01) （CSBT-WG-2019-01）
